特发性弱精子症精子与正常精子蛋白质双向电泳图谱分析  

Differential Analysis of Two-dimensional Gel Electrophoresis Profiles of Spermatozoal Protein in Human Normal Semen and Idiopathic Asthenospermia

作　　者：申树林[1] 罗勇[1] 宁亮[1] 贺大林[1] 

机构地区：[1]西安交通大学医学院第一附属医院泌尿外科,陕西西安710061

出　　处：《中华男科学杂志》2007年第1期50-52,共3页National Journal of Andrology

摘　　要：目的:探讨双向电泳技术在人类精子差异表达蛋白研究中的应用。方法:运用固相pH梯度双向凝胶电泳分离4例正常人和4例弱精子症患者精液标本的总蛋白质,凝胶银染后,用PDQuest软件进行分析,分辨出差异表达蛋白。结果:按照5倍的表达量计算,发现有差异表达的蛋白质点7个,2个点在弱精子症精子中高表达,而在正常精子为低表达;相反,在正常精子中高表达的5个点,而在弱精子症为低表达。结论:初步建立了正常精子和弱精子症患者精子的双向电泳图谱,并发现两者之间存在一些差异表达蛋白,为进一步研究特发性弱精子症精子差异表达蛋白的分离及鉴定奠定了良好基础。Objectives： To evaluate the application of two-dimensional electrophoresis in the research of differentially expressed proteins in human spermatozoa. Methods： Two-dimensional gel electrophoresis was performed on 4 sperm samples from normal healthy men and another 4 from asthenospermia patients. After silver staining, the differential expression proteins were analyzed by PDQuest 2D analysis software. Results： Seven differential protein spots were identified, 2 expressed highly in the asthenospermia sperm but lowly in the normal spermatozoa, while the other 5 expressed just the opposite way. Conclusion： The protein profiles of differential expression between the normal spermatozoa and idiopathic asthenospermia were established and some differential proteins were found. The data obtained in this study may help prepare the ground for further studies on the isolation and identification of differentially expressed proteins in human asthenospermia sperm.

关 键 词：特发性弱精子症 双向电泳 蛋白质 

分 类 号：R698.2[医药卫生—泌尿科学]