砷剂对角质形成细胞CDK2、CDK4和Cyclin-E蛋白表达的影响  

Effects of arsenic on CDK2,CDK4 and Cyclin-E expression in human epidermal keratinocytes

作　　者：毕新岭[1] 赵海生[2] 顾军[1] 

机构地区：[1]第二军医大学长海医院皮肤科,上海200433 [2]青海医学院附属医院皮肤科,西宁810001

出　　处：《中国麻风皮肤病杂志》2007年第1期4-5,共2页China Journal of Leprosy and Skin Diseases

摘　　要：目的:研究砷剂对角质形成细胞细胞周期调控因子蛋白表达的影响。方法:以体外培养的角质形成细胞株为对象,一定浓度砷剂处理细胞后,用免疫印迹方法研究HaCaT细胞周期蛋白依赖激酶2(CDK2)、周期蛋白依赖激酶4(CDK4)和细胞周期蛋白E(Cyclin-E)的表达情况及砷剂对其表达的影响。结果:未受到砷剂处理的对照组细胞稳定表达CDK2、CDK4和Cyclin-E,当1 nmol/L三氧化二砷处理24h后,HaCaT细胞表达CDK4、Cyclin-E水平升高,但CDK2的表达水平无明显变化。结论:一定浓度砷剂可刺激角质形成细胞增殖,可能与其升高细胞周期调控因子CDK4、Cyclin-E蛋白表达,导致S期细胞比例升高有关。Objective： To determine the effect of arsenic on the expression of CDK2, CDK4 and cyclin- E in human epidermal keratinocytes. Methods： Human epidermal keratinocytes （cell line HaCaT） were cultured in vitro. After treated with certain concentration of arsenic, the expressions of CDK2, CDK4 and cyclin- E were measured by immunoblot test. Results： Comparing with control group, the expression of CDK4 and cyclin - E was increased significantly when cells were treated by 1 nmol/L arsenic trioxide for 24 h, while there was no increase in the expression of CDK2. Conclusion： Certain concentration of arsenic may stimulate proliferation of HaCaT cell, which may be associated with increasing expression of CDK4 and cyclin- E, and the higher percentage cells in S phase.

关 键 词：砷剂 角质形成细胞 细胞周期 

分 类 号：R751[医药卫生—皮肤病学与性病学]