显齿蛇葡萄总黄酮对兔口腔黏膜溃疡的作用  

Effects of flavones from Ampelopsis grossedentata on oral ulcer of rabbits

作　　者：陈立峰[1] 陈莉萍[1] 徐琳本[1] 王晓洪[1] 

机构地区：[1]湖南省中医药研究院中药研究所,湖南长沙410013

出　　处：《中国药理学与毒理学杂志》2007年第1期49-54,共6页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的研究显齿蛇葡萄总黄酮抗口腔黏膜溃疡的作用。方法分别采用表皮葡萄球菌或10%乙酸注射到新西兰兔颊黏膜,或用同种异体口腔黏膜匀浆上清液作为免疫抗原注射于兔背部皮下,制备3种口腔黏膜溃疡模型。显齿蛇葡萄总黄酮剂量为84,266和840mg.kg-1,西地碘为1mg.kg-1,每天分4次口腔局部涂布给药3～4d。观察溃疡发生情况和溃疡愈合时间,检查溃疡直径和局部炎症指数,检测抗口腔黏膜抗体滴度和局部病理改变。结果显齿蛇葡萄总黄酮能使表皮葡萄球菌性、乙酸性口腔黏膜溃疡模型的溃疡直径明显缩小,炎症指数降低,愈合时间缩短,并随药物剂量增加作用增强;对免疫性口腔黏膜溃疡,可使溃疡发生率明显降低,抗体滴度下降,局部病理变化明显减轻。结论显齿蛇葡萄总黄酮有减轻口腔黏膜溃疡炎症、促进溃疡愈合的作用。AIM To vones from Ampelopsis verify the effects of flacer. METHODS Three models of oral ulcer were established by injection of Staphylococcus epidermidis, 10% acetic acid into rabbit buccal mucosa and supernate of homogeneous oral mucosa as an immunogen into rabbit back subcutaneously, respectively. The dosages of flavones were 84,266 and 840 mg. kg^-1 and cydiodine 1 mg. kg^-1, which were divided into 4 times a day and were daubed over and around buccal mucosa ulcer, respectively, for 3- 4 d. The ocurrence and healing time of oral ulcer were observed, the ulcer diameter and inflammation index around ulcer were measured, the pathological changes were observed under the microscope, and the titer of anti-oral mucosa antibody in sera was detected by turbidimetry. RESULTS The flavones significantly reduced ulcer diameter,lessened inflammation index, and shortened healing time of oral ucler induced by S. epidermidis and 10% acetic acid. In addition the flavones markedly decreased the rate of ulcer occurrence, slightly lowered the titer of antibody to homogeneous oral mucosa, and remarkably lightened the pathological changes in oral ucler induced by immunogen in rabbits. CONCLUSION The flavones from A. grossedentata inhibits inflammatory reaction of mucosa around oral ulcer and promotes the healing of oral ulcer.

关 键 词：显齿蛇葡萄 黄酮类 口腔黏膜 口腔溃疡 

分 类 号：R285.5[医药卫生—中药学]