一种从鸟类剥制标本提取DNA的改进方法  

An Improved Method for DNA Extraction from Avian Specimens

作　　者：吴唤玲[1] 韩德民[1] 章敬旗[1] 李进华[1] 

机构地区：[1]安徽大学生命科学学院安徽省生态工程与生物技术重点实验室,合肥230039

出　　处：《动物学杂志》2007年第1期84-88,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金资助(No.30470258)

摘　　要：应用非损伤性取样的方法,收集鸟类剥制标本的皮肤组织和羽毛,用无水乙醇、浸泡液预处理的方法抽提DNA,结果两者都可提取DNA供PCR扩增。将PCR产物经序列测定和比对分析,证明提取的DNA为目的DNA,表明本试验方法可行。鸟类剥制标本的皮肤组织和羽毛可以作为研究种群遗传学的资源。Non-invasive sampling is a useful tool for genetic analysis of endangered species, but it is often inapplicable due to the low quality and quantity of DNA obtained. In order to explore a method for extracting DNA from specimen of bird, we tried to extract DNA from dried skin of birds. We extracted total DNA of the koklass specimens successfully. Compared with the existing DNA extracting methods, our method allows the use of minute amount of dried skin and the feather,and this method is easier, quicker and more economical. The specimens of the dried skin and feathers are proved to be a good source of DNA extraction for genetic analysis.

关 键 词：鸟类 剥制标本 DNA提取 勺鸡 皮肤组织 

分 类 号：Q953[生物学—动物学]