大鼠心肌缺血后心肌组织端粒酶的表达变化  

THE EXPRESSION OF TELOMERASE IN MYOCARDIAL TISSUE AFTER MYOCARDIAL ISCHEMIA IN RAT

作　　者：任明芬[1] 郭志坤[2] 

机构地区：[1]新乡医学院第二附属医院心内科,新乡453002 [2]新乡医学院第二附属医院心脏形态学研究室,新乡453002

出　　处：《解剖学报》2007年第1期89-92,共4页Acta Anatomica Sinica

基　　金：河南省自然科学基金资助项目(412000900)

摘　　要：目的探讨盐酸异丙肾上腺素和冠状动脉结扎术致大鼠心肌缺血后心肌组织端粒酶(TMA)的表达。方法60只SD大鼠随机分为A、B、C 3个实验组,每组均设对照组。实验组经腹腔注射盐酸异丙肾上腺素8mg/kg后、随机分为A组(缺血30min组)和B组(缺血60min组),A、B对照组腹腔注射8ml/kg注射用水。C组为冠状动脉结扎30min组,3组均查心电图,左心室取血查心肌酶。组织学和免疫组织化学方法观察心肌组织TMA的表达,计算机图像分析测定TMA含量的变化。结果A、B、C 3个实验组TMA的表达均较对照组增强。计算机图像分析测定TMA的含量均较各自对照组升高,且具有显著的统计学意义(P<0.05或P<0.01)。3个实验组之间比较有显著的统计学差异。结论心肌缺血能刺激心肌组织TMA的表达增强。Objective To study the expression of telomerase（TMA） in myocardial tissue of myocardial ischemia in rat. Methods Sixty Spraque-Dawley rats were randomly divided into A, B, C three groups, then each group was randomly divided into control group and testing group.Two myocardial ischemia groups were injected isoprenaline （8 mg/kg）, and two control groups were injected distilled water （8 mg/kg） in abdominal cavity. For the last myocardial ischemia group the coronary artery was llgated. The histological and immunohistochemical method were used for observing cardiac structural changes, with HE staining,and changes of TMA in myocardial tissue after myocardial ischemia in rat. The TMA contents were measured by image analysis. Results Compared with the respective control group, the expression of TMA in the three myocardial ischemia groups increased obviously（ P 〈0.05 or P 〈 0.01）. The TMA contents in the three myocardial ischemia groups measured by image analysis were increased obviously,and had a prominent significance in statistics. Conclusion The TMA expression of rat myocardial tissue was increased after cardiac ischemla.

关 键 词：端粒酶 盐酸异丙肾上腺素 心肌缺血 免疫组织化学 大鼠心脏 

分 类 号：R332.1[医药卫生—人体生理学]