Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells  

作　　者：CHEN Zhiling Yu Yi LIU Lina XIA Guixian 

机构地区：[1]College of Life Science, Capital Normal University, Beijing 100037, China [2]Institute of Microbiology, Chinese Academy of Sciences, Beijiog 100080, China [3]These authors contributed equally to this work

出　　处：《Progress in Natural Science:Materials International》2007年第2期156-162,共7页自然科学进展·国际材料（英文版）

基　　金：Supported by the National Basic Research Priorities Program (Grant No. 2002CCA03000) ; National Natural Science Foundation of China (Grant No. 30100091)

摘　　要：The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48. The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase roodules （aa 245--374 and aa 518--646）. Overexpresslon of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through G1 to M phases, yet their subcenular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells.分裂酵母 Schizosaccharomyces pombe 被用来从可以在房间周期规定起作用的烟草 BY-2 房间识别基因。编码对酵母 CDC48 相应的蛋白质的 cDNA 被孤立，基因被指定为 NtCDC48。cDNA 包含为二个 twical ATPase 模块包括的 808 氨基酸的预言的蛋白质编码的一个开的读物框架(一 245-374 并且一 518-646 ) 。NtCDC48in 烟草 BY-2 房间的 Overexpression 在有丝分裂的索引以及到传播有丝分裂的锭子的形成导致了增加。NtCDC48-GFP 熔化蛋白质还通过 G1 无所不在地被散布到 M 阶段的家他们细胞的本地化与房间周期前进一起定期改变了的潜水艇。这些结果显示 NtCDC48 可以在 BY-2 房间在房间周期的规定起一个重要作用。

关 键 词：tobacco BY-2 suspension cells NtCDC48 SPINDLE cell cycle. 

分 类 号：S572[农业科学—烟草工业]