Cloning of the full-length gene of activin receptor-interacting protein2a and characterization of its interaction with ActR Ⅱ A  

作　　者：CUI Xueling TAI Guixiang ZHANG Hongjun FANG Lin LIU Zhonghui 

机构地区：[1]School of Basic Medical Sciences, Jilin University, Changchun 130021, China

出　　处：《Progress in Natural Science:Materials International》2007年第2期172-176,共5页自然科学进展·国际材料（英文版）

基　　金：Supported by National Natural Science Foundation of China (Grant Nos. 30170298 and 30571688)

摘　　要：To investigate the mechanism of intracellular signal transduction mediated by activin receptors, the full-length gene encoding a novel activin receptor-interacting protein2a （ARIP2a） was identified from a mouse brain cDNA library. The sequences of ARIP2a andARIP2, distribution of ARIP2a and ARIP2 mRNA in mouse tissues, and expression of ARIP2a and ARIP2 in activin-induced RAW264.7 cell were compared, and the interaction between ARIP2a and ActRIIA was confirmed. The sequence analysis revealed that the full-length gene of ARIP2a, which composed of 1008 bp and encoded 153 amino acid residues, shared high sequence identity with ARIP2 except the position of the 99th amino acid. RT-PCR assay showed that ARIP2a mRNA was highly expressed in brain, pituitary and testis, and moderately in pancreas and ovary, but undetectable in other tissues. Whereas, ARIP2 mRNA was widely distributed in all mouse tissues that we tested. Moreover, expression of ARIP2a mRNA was significantly decreased in activin-stimulated RAW264.7 cells; however, the expression of ARIP2 mRNA was increased. Additionally, the interaction between ARIP2a and activin type IIA receptor （ActRIIA） was further demonstrated by mammalian two-hybrid assays and pull-down assays. Taken together, those results indicate that although ARIP2a is homologous to ARIP2, they are different in tissue distribution and responses to activin. ARIP2a could also interact with activin type II receptor as a novel member of ARIP family.为了调查细胞内部的信号 transduction 的机制，由 activin 受体调停了，编码新奇 activin 的全身的基因交往受体的 protein2a (ARIP2a ) 从一个老鼠大脑 cDNA 图书馆被识别。ARIP2a 和 ARIP2 和在导致 activin 的 RAW264 的 ARIP2 的序列。7 房间被比较，并且在 ARIP2a 和 ActRIIA 之间的相互作用被证实。序列分析表明除了 99 <SUP 的位置， ARIP2a 的全身的基因， 1008 bp 创作了并且编码了 153 氨基酸残余，与 ARIP2 分享了高顺序 indentity > th </SUP> 氨基酸。RT-PCR 试金证明 ARIP2a mRNA 高度在大脑，垂体和睾丸被表示，并且中等在胰和卵巢，但是在另外的纸巾无法发现。而， ARIP2 mRNA 广泛地在我们测试了的所有老鼠纸巾被散布。而且， ARIP2a mRNA 的表示显著地在刺激 activin 的 RAW264 被减少。7 个房间；然而， ARIP2 mRNA 的表示被增加。另外，在 ARIP2a 之间的相互作用和 activin 类型 IIA 受体(ActRIIA ) 被哺乳动物的二混血儿的试金和下拉试金进一步表明。总起来说，那些结果显示尽管 ARIP2a 对 ARIP2 相应，他们在到 activin 的织物分发和回答是不同的。ARIP2a 能也与 activin 类型 II 受体(ActRIIA ) 交往被哺乳动物的 two-hybird 试金和下拉试金进一步表明。总起来说，那些结果显示尽管 ARIP2a 对 ARIP2 相应，他们在到 activin 的织物分发和回答是不同的。ARIP2a 能也作为 ARIP 家庭的一篇小说与 activin 类型 II 受体交往。

关 键 词：ACTIVIN receptor-interacting protein tissue distribution. 

分 类 号：Q25[生物学—细胞生物学]