Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus  

作　　者：DONG Zhi GUO Xiaoyu ZHANG Hailong ZHANG Feiyun 

机构地区：[1]College of Life Science, Capital Normal University, Beijing 100037, China [2]College of Resources Environment and Tourism, Capital Normal University, Beijing 100037, China [3]Beijing Key Laboratory for Resources Environment and Geography Information System, Beijing 100037, China [4]These authors contributed equally to this work

出　　处：《Progress in Natural Science:Materials International》2007年第2期222-225,共4页自然科学进展·国际材料（英文版）

基　　金：Supported by National Natural Science Foundation of China (Grant No. 30571010); Key Program of Scientific and Technological Research of Ministry of Education (No. 204006)

摘　　要：The coat protein （CP） gene of Cocksfoot mottle virus （CfMV） was amplified by RT-PCR and inserted into expression vector pGEX-4T-1, and the resulting plasmid was designated as pGEXCfMV-JANCP. The fusion protein GST-CP was expressed in BL21 （DE3） pLysS after IPTG induction. The results of SDS-PAGE and Western blot analysis showed that the CfMV-CP gene was efficiently expressed in E. coli BL21 （DE3） pLysS through IPTG induction and the 56.0 kD protein was obtained.公鸡脚杂色病毒(CfMV ) 的上衣蛋白质(CP ) 基因被 RT-PCR 放大并且插入了到表示向量 pGEX-4T-1，和产生原生质标志被指定为 pGEXCfMV-JANCP。熔化蛋白质 GST-CP 在 IPTG 正式就职以后在 BL21 (DE3 ) pLysS 被表示。SDS 页和西方的污点分析的结果证明 CfMV-CP 基因高效地在 E 被表示。通过 IPTG 正式就职和 56.0 kD 蛋白质的 coliBL21 (DE3 ) pLysS 被获得。

关 键 词：Cocksfoot mottle virus sequence analysis prokaryotic expression. 

分 类 号：S543.3[农业科学—作物学]