全氟化碳雾化吸入对兔急性肺损伤肺组织结构及抗炎作用的影响  

Effect of aerosolized perfluorocarbon on the tumor necrosis factor-α mRNA expression and lung histopathology in rabbits with acute lung injury

作　　者：黄志坚[1] 李琦[1] 王长征[1] 钱桂生[1] 宋鹏[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所,重庆400037

出　　处：《中国呼吸与危重监护杂志》2007年第1期45-48,I0003,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：全军"十一五"计划课题(编号:06G083)

摘　　要：目的观察雾化吸入全氟化碳(PFC)对急性肺损伤(ALI)兔肺组织病理学变化、气体交换及肿瘤坏死因子-α(TNF-α)mRNA表达的影响。方法用油酸制备兔ALI模型后,随机分为常规机械通气(CMV)组和PFC组,每组8只动物。分别在ALI前,ALI后,以及治疗后1,2,3和4h共6个时相点观察动脉血气指标的变化,实验结束后取6个不同部位的肺组织做病理切片,取2个不同部位的肺组织做TNF-αmRNA原位杂交。结果PFC组PaO2及肺静态顺应性(CLst)显著高于CMV组;PFC组病理改变轻于CMV组。原位杂交提示PFC组TNF-αmRNA原位表达低于CMV组。自身比较发现,背侧肺叶TNF-αmRNA表达重于腹侧肺叶(P<0.05)。结论雾化吸入PFC能明显改善实验兔ALI肺氧合能力,升高PaO2,改善CLst,减少肺组织中的TNF-αmRNA表达。Objective To investigate the effect of aerosolized perfluorocarbon （PFC） （FC77） on gas exchange,histopathological changes of lung in acute lung injury and pulmonary expression of tumor necrosis factor-α （TNF-α） mRNA.Methods After acute lung injury （ALI） was induced by oleie acid （OA） ,16 rabbits were assigned nmdomly into 2 groups, ie. aerosolized perfluorocarbon group （ PFC group） and conventional mechanical ventilation group （CMV group）. Gas exchange parameters were measured before and after ALI, at 1, 2,3,4 h after treatment. Histological sections taken from 6 different parts of lung were stained by hematoxylin and eosion. The express of TNF-α mRNA in the 2 different parts of lung were detected by in situ hybridization （LSH）. Results Compared with CMV group, the PaO2 and static lung compliance （CLst） were significantly increased （P 〈0.05）,the histopathological lesions of lung were attenuated, and the TNF-α mRNA expression was decreased significantly in PFC group （ all P 〈 0.05）. There was more expression of TNF-α mRNA in backside than that in foreside of lung in two groups （ P 〈 0.05）. Conclusion Aerosolized perfluocarbon （PFC） can decrease expression of tumor necrosis factor-α mRNA in the lung, and improve the CLst and oxygenation during lumg injury.

关 键 词：急性肺损伤 全氟化碳 病理 肿瘤坏死因子-Α 

分 类 号：R563.8[医药卫生—呼吸系统]