高效液相色谱法测定人血浆罗哌卡因的浓度  

Determination of the concentration of ropivacaine in patients by high performance liquid chromatography

作　　者：冉菊红[1] 马民玉[1] 李国栋[2] 

机构地区：[1]郑州大学第一附属医院麻醉科,郑州450052 [2]郑州大学医学院实验中心药理实验室

出　　处：《中原医刊》2007年第3期3-4,共2页Central Plains Medical Journal

基　　金：河南省科技攻关资助项目（0211043900）

摘　　要：目的 建立测定人血浆罗哌卡因浓度的高效液相色谱方法。方法 LC-6A高效液相色谱仪，色谱柱为Hy—persilC18（150mm×4．6mm，5μm）。以布比卡因为内标，流动相采用乙腈：水=17：83（V／V），流速2．0ml／min，在紫外检测波长210nm处进行检测。结果 本方法标准曲线线性方程为Y=1．1236X＋0．0245（y=0．9997，线性浓度范围为0．025～2．5mg／L）；最低检测限为0．0125mg／L；平均方法回收率为99．63％；日间、日内精密度RSD均〈9％。结论 本方法简便、灵敏、快捷、准确，可用于临床上罗哌卡因血药浓度的监测和药代动力学研究。Objective To establish a high performance liquid chromatography （HPLC） method for determination of the concentration of ropivacaine in human plasma. Methods LC - 6A HPLC instrument was used with the column of Hypersil C18（ 150 mm ×4.6 mm,5μm）. Internal standard was bupivacaine. The mobile phase composed of acetonitrile -water was 17：83 （V/V）. The flow rate was 2.0 ml/min. The fluorescence detective wave length was 210 nm. Results The standard curve equation was Y = 1. 1236X ＋ 0. 0245 （ y = 0. 9997）. The linear range was 0. 025 -2. 5 mg/L. The limit of detection was 0. 0125 mg/L. The average recovery was 99.63%. RSD of inter - day and intra - day precision（ RSD% ） were all less than 9%. Conclusion The HPLC method is simple, accurate and sensitive for monitoring ropivacaine concentration in clinical plasma samples and pharmacokinetics study.

关 键 词：色谱法 高效液相 罗哌卡因 血浆 

分 类 号：O65[理学—分析化学] R9[理学—化学]