RP-HPLC法同时测定人血清中内源性氢化可的松与可的松的含量  

Simultaneous Determination the Contents of Cortisol and Cortisone in Serum by HPLC

作　　者：陈荣[1] 邹素兰[1] 薛晖[1] 钱爱而[1] 谢陶吟[1] 

机构地区：[1]常州市第一人民医院,江苏常州213003

出　　处：《中国药师》2007年第2期107-109,共3页China Pharmacist

基　　金：江苏省卫生厅临床药学科研项目(编号:P200504)

摘　　要：目的:建立测定人血清中内源性氢化可的松与可的松含量的方法。方法:采用迪马Diamonsil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相:乙腈-水-甲醇(51:136:77);流速:1.2 ml·min^(-1);紫外检测波长:245 nm;柱温:30℃;进样量:30μl。结果:氢化可的松在50.0～300.0μg·L^(-1),可的松在10.0～100.0μg·L^(-1)的浓度范围内线性关系良好。氢化可的松与可的松的方法回收率分别为(101.83±1.19)%和(100.52±5.91)%。92名健康者血清中氢化可的松、可的松的浓度分别为(111.60±27.30)和(18.66±4.44)μg·L^(-1),其比值为(6.08±1.10)。结论:本法操作简便,灵敏度高,快速可靠,适用于氢化可的松与可的松含量的测定,为研究人体肝脏11β-羟基类固脱氢酶(11β-HSD)的活性及调整激素治疗方案提供有效的分析手段。Objective： To establish a HPLC method for determination the contents of cortisol and cortisone in human serum. Method： The Diamonsil Cts column （250 mm ×4.6 mm,5μm ） was Used and the mobile phase was acetonitrile-water-methanol （51： 136： 77）, and flow rate was 1.2 ml·min^-1, detected wavelength was at 245 nm, and the column temperature was 30℃, the sampling volume was 30 μl. Result： F and E in serum solution were well separated. The linear correlation coefficient of cortisol calibration curve was 0. 999 4 within the range of 50.0 - 300μg·L^ -1, and the average recovery was（ 101.83 ±1.19） % . The linear correlation coefficient of cortisone calibration curve was 0.99.7 4 within the rang of 10.0 -100μg·L^-1 and the average recovery was（ 100.52±5.91 ）%, respectively. The levels of cortisol and cortisone in the serum of 92 healthy volunteers were（ 111.60± 27.30）μg·L^-1 and （18.44 ± 4.44）μg·L^-1, respectively. Cortisol/cortisone was 6.08 ± 1.10. Conclusion： The methods are simple, accurate, stable and suitable for measuring human serum glucocorticoid.

关 键 词：高效液相色谱法 氢化可的松 可的松 11β-羟基类固脱氢酶 

分 类 号：R97[医药卫生—药品]