甲泼尼龙对人肝癌裸鼠皮下移植瘤生长的影响  

Effect of methylprenisolone on the growth of hepatocellular carcinoma in nude mice

作　　者：曾纪晓[1] 何晓顺[1] 马毅[1] 朱晓峰[1] 韩明[1] 张珑涓[2] 史成军[3] 

机构地区：[1]中山大学附属第一医院器官移植中心,广州510080 [2]中山大学附属第一医院外科实验室,广州510080 [3]中山大学肿瘤防治中心

出　　处：《中华实验外科杂志》2007年第2期190-192,共3页Chinese Journal of Experimental Surgery

基　　金：广东省教育厅“千百十工程”人才基金项目（Q02033）;广州市科技攻关计划资助项目（200523-E0041）;China Medical Board in NewYork（06837）

摘　　要：目的探讨甲泼尼龙（MP）对人肝癌裸鼠皮下移植瘤生长的影响及机制。方法建立人肝癌裸鼠皮下移植瘤模型20只，随机分为对照组、MP小剂量组、MP大剂量组。用药4周后观察裸鼠重量、肿瘤体积和重量、肿瘤组织切片透射电镜检查、放射免疫法测定血AFP浓度、荧光定量聚合酶链反应（PCR）检测肿瘤组织血管内皮生长因子（YEGF），、基质金属蛋白酶2（MMP-2）的表达。结果MP大剂量组肿瘤重量（1．62±0．12）Vs（1．33±0．19）g、体积（0．63±0．05）Vs（0．56±0．05）cm^3大于对照组，该组裸鼠血AFP浓度高于对照组（7．5±1．14Vs（5．98±0．78）μg/L，VEGF mRNA、MMP-2mRNA表达显著增加。结论MP大剂量可以上调VEGF mRNA、MMP-2mRNA的表达，促进肝癌细胞生长、转移，小剂量无影响。Objective To study the effect of methylprenisolone on the hepatocellular carcinoma and the relevant mechanism. Methods Twenty nude mice bearing subcutaneous xenograft human hepatocellular carcinoma （HCC） were randomly divided into 3 group： control group, low -dose group and high - dose group. The effect of methylprenisolone was evaluated after 4 weeks, including the weight of nude mice, the tumor volume and weight. The blood concentration of AFP was measured by radioimmunoassay. The expression of VEGF and MMP-2 was detected by fluorescent quantitative polymerase chain reaction （FQ-PCR）. Results Tumor weight （ 1.62 ± 0.12） g vs （ 1.33 ± 0.19） g and volume （0.63 ± 0, 05） vs （0.56 ± 0.05） cm^3 were significantly increased in high -dose group. The blood concentration of AFP was augmented in high -dose group （7.50 ± 1.14） vs （5.98 ±0.78） μg/L. The expression of VEGF and MMP-2 was up -regulated significantly in high -dose group, Conclusion The growth and metastasis in the nude mice were enhanced when exposed to high dose of methylprenisolone. The mechanism attributes to the up -regulation of VEGF and MMP-2 expression probably.

关 键 词：癌 肝细胞 血管内皮生长因子 基质金属蛋白酶 

分 类 号：R686[医药卫生—骨科学]