分化抗原簇14基因多态性与感染易感性的相关研究  

Association between cluster of differentiation 14 polymorphism and susceptibility of infection

作　　者：冷伟建[1] 宋勇[1] 于宝军[2] 施毅[1] 

机构地区：[1]南京军区南京总医院、南方医科大学临床学院呼吸科,江苏210002 [2]南京军区南京总医院普外科实验室

出　　处：《中国危重病急救医学》2007年第1期17-20,共4页Chinese Critical Care Medicine

基　　金：教育部留学回国人员科研启动基金（2003-14）

摘　　要：目的 通过检测分化抗原簇14基因（CD14）单核苷酸多态性（SNP）的频率分布．探讨基因多态性与感染易感性之间的关系。方法选择2004年12月-2006年3月进入重症加强治疗病房（ICU）并发全身性感染的患者112例，并以100名健康自愿献血者为正常对照组。采用聚合酶链反应（PCR）扩增限制性内切酶长度多态性（RFLP）的方法检测CD14基因上-159C／T及-260C／T两个位点的SNP。结果全身性感染组与正常对照组比较：两组CD14-159基因型分布差异有显著性（P=0．005），全身性感染组CD14-159的T等位基因出现频率显著高于正常对照组（P=0．000）；两组CD14-260基因型分布及等位基因出现频率差异均无显著性（P=0．912和P=0．706）。死亡组与存活组比较：两组CD14-159基因型分布差异有显著性（P=0．000），死亡组CD14-159的T等位基因出现频率显著高于存活组（P=0．000）；CD14-260基因型分布及等位基因出现频率在两组问差异均无显著性（P=0．643和P=0．890）。结论CD14基因-159C／TSNP的差异可能与全身性感染患者易感性及感染后病死率增高有关。Objective To investigate the （SNPs） of cluster of differentiation 14 （CD14） association between the single nucleotide polymorphisms and susceptibility in patients with sepsis, Methods One hundred and twelve patients with sepsis admitted to the intensive care unit （ICU） from December 2004 to March 2006 were enrolled for study. Another 100 healthy volunteer donors were chosen serving as the normal control group. Genotypes and alleles of CD14 - 159 C/T, CD14 - 260 C/T were determined in patients and healthy controls by means of restriction fragment length polymorphism （RFLP） analysis of polymerase chain reaction （PCR） products. Distributional differences of genotypes and alleles between patients and healthy controls were analyzed. The sepsis patients were divided into survivor group and nonsurvivor group depending on survival for 28 days. Distributional differences of genotypes and alleles between survivor group and nonsurvivor group were determined. Results The rates of CD14 - 159 C/T genotypes and T- alleles in patients with sepsis were significantly higher than those of healthy volunteers （P= 0. 005 and P=0. 000）. There were no significant differences in the rates of CD14- 260 C/T genotypes and T-alleles in two groups （P=0.912 and P= 0. 706）. The rates of CD14 - 159 C/T genotypes and T - alleles in nonsurvivors were significantly higher than those of survivors （both P = 0. 000）. There were no significant differences in the rates of CD14 -260 C/T genotypes and T -alleles in two groups （P= 0. 643 and P=0. 890）. Conclusion The differences of CD14 159 C/T genotypes of SNPs may be associated with the higher risk of susceptibility and mortality of sepsis.

关 键 词：分化抗原簇14基因 基因多态性 全身性感染 

分 类 号：R686[医药卫生—骨科学]