FK506缓释膜应用于同种异体神经移植的实验研究  

Application of FK506 sioworelesing film in peripheral nerve alintransplantation： an experimental study in mice

作　　者：张桂生[1] 于晋辉[1] 邵新中[1] 张利民[1] 

机构地区：[1]河北医科大学附属第三医院手外科,石家庄050051

出　　处：《中华手外科杂志》2007年第1期8-10,共3页Chinese Journal of Hand Surgery

摘　　要：目的 研究普乐可复（prograf，FK506）缓释膜在同种异体神经移植中的作用。方法 应用异体神经桥接大鼠坐骨神经缺损，术中局部应用FK506缓释膜，分别于术后4、8、12周对移植物行大体和光镜观察，及轴突图像分析、移植神经电镜检查、小腿三头肌肌湿重比较、患肢坐骨神经电生理检查。结果 C组（应用FK506缓释膜）的神经生长最好，基本与D组（自体神经移植）相同，B组（经预处理异体神经移植）次之，A组（新鲜异体神经移植）最差。经过对肌电图、轴突计数、肌湿重的统计学分析，C、B、A组的差异有统计学意义（P〈0．05），C、D组之间差异无统计学意义。结论 应用FK506缓释膜有助于减轻同种异体神经的免疫排斥反应，为神经再生创造良好条件；在同种异体神经移植中应用FK506缓释膜有助于促进神经再生。Objective To study peripheral nerve regeneration after sciatic nerve allotransplantation and the effect of FK506 slow-releasing film. Methods Sciatic nerve allotransplantation model was created in mice. FK506 slow-releasing film was applied near the nerve. At the 4th, 8th and 12th weeks postoperatively, gross observation of the graft, electrophysiolngical examination, gastrocnemius muscle wet weight, histology （HE, silver staining, S-100 protein）, neuromorphometry, and electron microscopic studies were carried out. Results The quality of nerve regeneration in group C （FK506 slow-releasing film group） was the best, being almost as good as that of group D （autologous nerve graft group）. Nerve regeneration was less ideal in group B （ preprocessed allngraft group） and worst in group A （fresh allograft group）. Statistical analysis of data from electromyngraphy, muscle weight and axon count showed that the difference between group C and group A, B was significant. There was no significant difference between groups C and D. Conclusion Application of FK506 slow-releasing film to nerve allograft can not only eliminate rejection reaction but also aceelerate peripheral nerve regeneration and functional recovery.

关 键 词：周围神经 神经再生 神经移位 普乐可复 

分 类 号：R686[医药卫生—骨科学]