多种免疫抑制剂对肾毒血清性肾炎大鼠肾组织中MMP-9的表达影响  

The effect of newer immunosuppressive on the expression of MMP-9 in nephrotoxic nephritis in rats

作　　者：江洁龙[1] 张伯科[1] 任伟[2] 王坤[1] 赵珉[1] 钱浩[1] 戴宏[1] 梁维龙[1] 阚春明[1] 

机构地区：[1]安徽医科大学第一附属医院肾内科,合肥230022 [2]安徽医科大学附属省立医院肾内科,合肥230001

出　　处：《安徽医科大学学报》2007年第1期17-20,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金资助项目(编号:04023051)

摘　　要：目的探讨免疫抑制剂霉酚酸酯、来氟米特和他克莫司对肾毒血清性肾炎大鼠肾组织中基质金属蛋白酶-9表达的影响。方法制备肾毒血清性肾炎大鼠模型,并随机分为病理对照组、霉酚酸酯治疗组、来氟米特治疗组和他克莫司治疗组,每组各10只。各治疗组均给予相应药物灌胃给药每天1次,2周后观察血清总蛋白(Tp)、白蛋白(Alb)、血肌酐(Scr)和尿素氮(BUN)水平。应用免疫组织化学观察肾组织中基质金属蛋白酶-9的表达。结果基质金属蛋白酶-9在病理对照组肾小球和肾小管中的表达均较正常对照组增高(P<0.01);与病理对照组比较,霉酚酸酯、来氟米特和他克莫司治疗后可使其表达减少(P<0.01),且来氟米特治疗组较其它两用药组下降明显(P<0.05)。各治疗组与病理对照组比较Tp、Alb增加(P<0.01),Scr、BUN降低(P<0.01)。结论霉酚酸酯、来氟米特和他克莫司治疗均能减少肾毒血清性肾炎大鼠肾组织中基质金属蛋白酶-9的表达,改善肾功能,其中来氟米特效果最理想。Objective To investigate the effects of immunosuppressive agents such as Mycohonolate mofetil （MMF） ,Leflunomide （LEF） and tarcrolimus （FKS06） therapy on the expression of MMP-9 in rats with Nephrotoxic Nephritis. Methods Nephrotoxic Nephritis was induced in the other 40 rats and these rats were randomly di- vided into four groups ： nephritis group, MMF therapy group, LEF therapy group and FKS06 therapy group. Each of the therapy groups was given intragastric administration once every day. After 2 weeks, serum Total Protein （ Tp）, Albumin （Alb）, Blood Urea Nitrogen （BUN） and Serum creatine （Scr） of every rat were determined. Immunohistochemistry was used to evaluate the change and expression of MMP-9 in the tissues of kidney. Results The ex- pression of MMP-9 in glumeruli and renal tubles in the nephritis group was much higher than that in nomal group （P 〈0. 01 ）, The expression in therapy groups was much lower than that in nephritis group（P 〈0. 01 ） ,and in LEF therapy group it was much lower than other groups （ P 〈 0. 05 ）. The thrapy groups had much higher Tp and Alb level and much lower BUN and Scr level than the nephritis group（ P 〈 0. 01 ）. Conclusion MMF, LEF, and FK506 reduces the expression of MMP-9 in the tissues of kidney, with preservation of renal function in experimental crescentic glomerulonephritis, meanwhile LEF has the best effect in three therapy groups.

关 键 词：霉酚酸 异恶唑类 他罗利姆 肾小球肾炎 明胶酶B 

分 类 号：R692.31[医药卫生—泌尿科学] R979.5[医药卫生—外科学]