原癌基因c-myc与血管内皮生长因子对血管成形术后再狭窄的影响  

Research antisence c-myc of and VEGF on restenosis after angioplasty

作　　者：杨予川[1] 陈颖[1] 梁晓辉 李晓夫[1] 杨署[1] 宋涛[3] 

机构地区：[1]哈尔滨医科大学附属第一医院放射科,150001 [2]哈尔滨市红十字中心医院放射科 [3]哈尔滨医科大学附属第一医院心内科

出　　处：《中国急救医学》2007年第1期49-51,共3页Chinese Journal of Critical Care Medicine

基　　金：黑龙江省自然科学基金(No.D0333)

摘　　要：目的探讨VEGF与下调c-myc联合干预对血管成形术后再狭窄的影响。方法中国大耳白兔28只,分为4组,每组7只；髂总动脉球囊拉伤后,分别给予反义c-myc和(或) VEGF,取病理切片对再狭窄程度进行评价；采取免疫组化及Western blotting对联合应用VEGF和反义c-myc效果进行评价。结果下调c-myc与VEGF均能减轻血管成形术后再狭窄,反义c-myc与VEGF联合应用则显著减轻再狭窄,但两者间无显著交互作用(P＞0．05)。结论联合干预c-myc与VEGF显著减轻再狭窄,但两者之间无协同作用。Objective To investigate the effect of downregulation c - myc and VEGF in combinat- ed applying on restenosis after angioplasty. Methods 28 China rabbits were divided randomly into four groups with each group containing 7 rabbits. After ball - injured, antisense c - myc or/and VEGF was performed. Group A as control, group B antisense c - myc respectively, group C VEGF only, group D an- tisense c - myc and VEGF. Evaluating on restenosis by pathological section, immunohistochemistry and Western blotting for the expressing of PCNA . Results Pathological section： （ intimal and media ） both downregulation c - myc and VEGF can relieve restenosis after angioplasty, combining antisense c - myc with VEGF can relieve restenosis more effectly. The situations of immunohistochemistry and Western blotting were on the analogy of pathological section. Conclusions Downregulation c - myc and VEGF has definite effect in preventing restenosis after angioplasty. Combining antisense c - myc with VEGF do not achieve concerted effect.

关 键 词：原癌基因 血管成形术 血管内皮生长因子 再狭窄 

分 类 号：R541.4[医药卫生—心血管疾病]