基因工程菌对阿特拉津的生物转化及其影响因素  

The Biotransformation of Atrazine and Influence Factors by Genetically Engineered Microorganism

作　　者：刘春[1] 黄霞[1] 王慧[1] 

机构地区：[1]清华大学环境科学与工程系环境模拟与污染控制国家重点实验室,北京100084

出　　处：《微生物学通报》2007年第1期10-14,共5页Microbiology China

基　　金：国家重点基础研究发展计划(973)项目(No.2004CB418506)

摘　　要：研究考察了基因工程菌转化阿特拉津的共代谢碳源、转化动力学和影响因素。结果表明,作为共代谢碳源,葡萄糖优于乙酸盐,碳源浓度对转化影响不大,对工程菌生长影响显著。阿特拉津比转化速率与工程菌初始密度无关,与阿特拉津初始浓度有关,用Monod方程拟合转化动力学,求得方程参数为V_(max)=0．168/h,Ks= 30．49mg/L。降低温度会显著降低阿特拉津比转化速率;偏碱性的条件下,阿特拉津转化率较高,酸性条件严重抑制阿特拉津转化;盐度在一定范围内不影响转化活性;Co^(2+)、Fe^(2+)、Fe^(3+)和Zn^(2+)促进阿特拉津转化,Mn^(2+)、Ni^(2+)和Cu^(2+)抑制阿特拉津转化。菌体细胞对阿特拉津的吸附和转化作用呈正相关关系。The co-metabolic carbon sources, dynamics and influence factors of atrazine biotransformation by genetically engineered microorganism （GEM） were investigated in the paper. The results showed that glucose was superior to acetate as co-metabelic carbon sources. The concentration of carbon sources affected little on atrazine transformation but much on GEM growth. The specific transformation rate （STR） of atrazine was influenced not by cell density of GEM but by atrazine concentration. Monod model was used to simulate atrazine transformation dynamics and the parameters were vmax = 0. 168/h and Ks = 30. 49 mg/L. When temperature reduced, STR decreased markedly. The transformation was efficient in weak alkaline condition and was inhibited in acidic condition. To some extent, salinity did not affect transformation activity of GEM. Co^2＋ , Fe^2＋ , Fe^3＋ , and Zn^2＋ improved atrazine transformation, but Mn^2＋ , Ni^2＋ and Cu^2＋ inhibited atrazine transformation. The results also showed that there existed a direct correlation between adsorption and transformation of atrazine by GEM.

关 键 词：基因工程菌 阿特拉津 生物转化 

分 类 号：X592[环境科学与工程—环境工程]