高抗体水平种鸡NDV的分离鉴定和分子特性  

Isolation,Identification and Molecular Characterization of a Velogenic NDV Isolate from a Broiler Flock in High Antibody Level Against NDV

作　　者：马保臣[1] 何叶峰[1] 徐怀英[1] 王友令[1] 秦卓明[1] 李莉[1] 欧阳文军[1] 

机构地区：[1]山东农业科学院家禽研究所

出　　处：《微生物学通报》2007年第1期95-100,共6页Microbiology China

基　　金：山东省科技攻关重点支持项目(No.030317);山东省自然基金支持项目(No.031020101)

摘　　要：从抗体水平较高、产蛋下降的种鸡群分离到一株新城疫病毒(Newcastle Disease virus,NDV)SGM01,经动物回归可产生NDV典型病变,其MDT、ICPI和IVPI等分别为50．5h、1．76和2．41,表明为强毒。对其F和HN基因进行克隆测序,F基因氨基酸多肽裂解位点的基序为^(111)GRRQKRF^(117),与强毒基序一致。基因分型表明SGMO1属Ⅶ型。氨基酸同源比较显示:SGM01与LaSota、SQZ04等基因Ⅱ型的同源性为:87．7%～88．3%;(?) Taiwan95、Yunnan03等基因Ⅶ型的同源性为:95．7%～98．2%;与F_(48)E_9等基因Ⅸ型的同源率为:90．8%～91．7%。HN基因与F基因不同,具有明显的时空性。SGM01、Taiwan95、SQZ04等新近分离毒氨基酸高度同源,同源性为:95．3%～97．2%,显著高于与LaSota(经典弱毒疫苗株)和F_(48)E_9(经典强毒株)的同源性87．4%～89．0%。A Newcastle disease virus （NDV） field strain SGM01 was isolated from a broiler flock in high antibody level against NDV, and identified by HA, HI cross test and animal regression. SGM01 was determined as a virulent strain with MDT of 50. 5h , ICPI of 1.76, IVPI of 2. 41 after plaque-purification. The F and HN gene of SGM01 were cloned and sequenced. Analysis of F gene indicated that SGM01 belonged to genotype Ⅶ. The amino acid sequence ^111GGRQGRL^117 in the F protein cleavage site in SGM01 strain is identical to virulent NDV. The homology analysis of F and HN gene sequences compared to reference strains from GenBank indicated that ： The F protein amino acid sequence has homologies of 87.7% - 88. 3% with published gene type Ⅱ strains LaSota and SQZ04 et al. , 95.7% - 98.2% with genotype Ⅶ strains Taiwan95, Yunnan03 et al. , 91.8% -91.7% with genotype Ⅸ strains F48E9 et al.. While HN genes were compared, SGM01 demonstrated a higher homologies of 96. 5% -97.2% with strains which were isolated in recent years , but lower homologies of 87. 4% and 89.0% with LaSota and F48E9.

关 键 词：新城疫病毒 高抗体水平 分子特性 

分 类 号：S852.65[农业科学—基础兽医学]