龙须菜体表附生细菌的几种分离方法比较  

Comparison with Several Methods to Isolate Epiphytic Bacteria from Gracilaria lemaneiformis (Rhodophyta)

作　　者：徐永健[1] 乐观宗[1] 张友平[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,宁波31521

出　　处：《微生物学通报》2007年第1期123-126,共4页Microbiology China

基　　金：宁波市博士基金项目(No.2005A610025);宁波市科技项目(No.2004610028);宁波市自然科学基金项目(No.2006A610087)

摘　　要：采用超声波粉碎法、涡旋振荡法、超声波清洗法、研磨匀浆法等4种不同的方法处理龙须菜，分离其体表的附生细菌，并对分离细菌的数量、种类、形态结构、细胞壁特性等进行了观察和分析。通过对不同方法和相同方法的不同处理所得结果比较显示，超声波清洗法和研磨匀浆法对分离细菌的数量和种类效果都较差；超声波粉碎法和涡旋振荡法效果较好，尤以超声波粉碎法的30W30s处理效果最好，该方法分离到本项目4个方法13个处理获得的16个菌株中的12个菌株，龙须菜的细菌数1．75×10^6cells／g。We used 4 methods, such as ultrasonic crash （UC）, ultrasonic rinse （UR） , whorl surge （WS） and rubbing （RU）, to isolate epiphytic bacteria from red alga Gracilaria lemaneiformis. Then, we counted bacteria numbers, detected bacterial species, observed bacterial configuration and characteristic of cell wall Compared with these methods and with different treatments in one method, the results were drawn： the UR and RU were inferior in all methods to isolate bacterial numbers and species, the UC and WS were better, especially, the treatment 30W 30s of UC was the best in experiment, which isolated 12 of 16 bacterial species, and got 1.75 104 cells per gram wet weight G. lemaneiformis.

关 键 词：海藻 龙须菜 附生细菌 分离方法 

分 类 号：Q93-33[生物学—微生物学]