鸡EX-FABP基因PCR-SSCP分析  

PCR-SSCP analysis of extracellular fatty acid binding protein gene in chicken

作　　者：王彦[1] 舒鼎铭[1] 杨纯芬[1] 瞿浩[2] 刘益平[2] 朱庆[2] 

机构地区：[1]广东省农科院畜牧研究所,广东广州510640 [2]四川农业大学动物科技学院,四川雅安625014

出　　处：《黑龙江畜牧兽医》2007年第2期13-16,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：广东省自然科学基金项目(2004BA51409-1)

摘　　要：以AA白鸡、广西鸡、E1矮小鸡品系为试验材料,采用PCR-SSCP技术对鸡细胞外脂肪酸结合蛋白基因(EX-FABP)的2个片段进行PCR扩增,分析了EX-FABP基因在3个鸡品系的多态性。结果表明:EX-FABP基因在2对引物扩增片段中均存在PCR-SSCP多态性。对于引物1扩增片段,3个鸡品系均检测到BB基因型,AA基因型只出现在AA白鸡中;而且B等位基因频率在3个鸡品系中均明显高于A等位基因频率。对于引物2扩增片段,3个鸡品系均检测到HH和Hh基因型,hh基因型只出现在E1矮小鸡中;H等位基因频率在3个品系中均明显高于h等位基因频率。引物1和引物2的多态性片段测序分析表明,EX-FABP基因第641位点和645位点分别发生了单碱基的转换(G-A和T-C),第3264位点发生了转换(T-C)。The polymorphism of Extracellular Fatty Acid Binding Protein （ EX - FABP） gene in AA breed, Guang Xi breed and E1 Ai Xiao breed was ana- lyzed by PCR- SSCP. The results indicated that there were three genotypes （AA, AB and BB） detected by primer 1 BB genotype was detected in all the three breeds. AA genotype was only detected in AA breed. Frequency of B allele was obviously higher than that of A allele in all the three breeds. There were also three genotypes （HH, Hh and hh） were detected by primer 2 HH and Hh genotype were detected in all the three breeds; hh genotype was only detected in El Ai Xiao breed. Frequency of H allele was obviously higher than that of h allele in all the three breeds. The polymorphie fragments amplified by both primers were cloned and sequenced. The sequencing results showed that there was transition（T-C） at 645 site and（G A）at 641 site,one transition（T- C） at 3 264 site of EX - FABP gene in chicken, respectively

关 键 词：鸡 EX—FABP基因 PCR—SSCP 

分 类 号：S858.31[农业科学—临床兽医学]