LC-MS/MS检测减肥保健品中的盐酸芬氟拉明和盐酸西布曲明  

Determination of fenfluramine and sibutramine hydrochloride in slimming foods by liquid chromatography-tandem mass spectrometry

作　　者：权伍英[1] 栾燕[1] 张欣[1] 

机构地区：[1]沈阳市疾病预防控制中心,沈阳110031

出　　处：《中国卫生检验杂志》2007年第1期10-12,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立中药减肥保健品中盐酸芬氟拉明和盐酸西布曲明的液相色谱-串联质谱分析方法。方法：以伪麻黄碱为内标，用甲醇提取，用Diamonsil C18色谱柱分离，流动相为甲醇-水-甲酸（体积比为70：30：0．2），液相色谱-电喷雾串联质谱法进行测定。结果：盐酸芬氟拉明和盐酸西布曲明的线性范围均为5-400ng／ml，平均回收率均在98．8％-120％范围内，相对标准偏差均在5％以下。盐酸芬氟拉明的最低检测浓度为0．05ng／ml，盐酸西布曲明的最低检测浓度为0．025ng／ml。结论：本法专属性强，灵敏度和准确度高，可快速检测减肥保健品中掺入的盐酸芬氟拉明和盐酸西布曲明。Objective： To develop a liquid chromatography - tandem mass spectrometry method for the determination of fenfluramine hydrochloride and sibutramine hydrochloride in herbal slimming products. Methods： The analytes and the internal standard, pseudoephedrine were extracted with methanol, chrematographed on a diamonsil C18 column with methanol- water- formic acid （70：30：0. 2） as mobile phase and detected by tandem mass spectrometry with electrospray ionization source. Results：The calibration curve for fenfluramine hydrochloride and sibutramine hydrochloride showed good linearity in the range of 5 - 400 ng/ The mean recovery ranged from 98. 8% to 120% and the relative standard derivations were lower than 5%. The limit of quantification for fenfluramine hydrochloride and sibutramine hydrochloride were 0. 05 ng/ml and 0. 025 ng/ml, respectively. Conclusion：The method is sensitive, specific and suitable for rapid determination fenfluramine and sibutramine hydrochloride illegal used in herbal slimming products.

关 键 词：液相色谱-串联质谱法 减肥保健品 盐酸芬氟拉明 盐酸西布曲明 

分 类 号：O657.63[理学—分析化学]