大鼠阴茎包皮和包皮系带内nNOS免疫阳性神经末梢的分布与起源  

The distribution and origin of nNOS immunoreactive nerve terminals in the penile frenulum of adult rats

作　　者：吴仲敏[1] 王东红[1] 

机构地区：[1]台州学院医学院解剖学教研室,台州318000

出　　处：《中国男科学杂志》2007年第1期19-22,共4页Chinese Journal of Andrology

基　　金：浙江省教育厅科研项目(NO:20060077)立项资助

摘　　要：目的观察成年SD大鼠阴茎包皮和包皮系带内神经型一氧化氮合酶(nNOS)免疫阳性神经末梢的分布和起源。方法免疫组织化学法观察nNOS免疫阳性神经末梢的分布,荧光金(Fluoro-gold,FG)逆行追踪和nNOS免疫荧光标记相结合法研究大鼠包皮系带内nNOS免疫阳性神经末梢的起源。结果成年SD大鼠阴茎包皮和包皮系带内均有nNOS免疫阳性神经末梢存在,这些神经末梢主要位于表皮基底层和真皮乳头层,呈树枝状或念珠状分布。阴茎系带处nNOS免疫阳性神经末梢的图像光密度值(3.4±0.38)明显大于阴茎包皮处(2.2±0.45)。FG逆标阳性细胞位于大鼠第六腰髓对应的背根神经节(L6-DRG)(9.6±1.2)个/mm2和第一骶髓对应的背根神经节(S1-DRG)(1.2±0.2个/mm2)内。阳性细胞大中小不等,大多沿神经束成行排列或散在分布。nNOS免疫荧光标记细胞在L6-DRG和S1-DRG内分别为(24.2±2.6)个/mm2和(24.1±2.1)个/mm2,细胞大多呈中小型。FG/nNOS双标阳性细胞均为中小型,其数量接近FG逆标阳性细胞总数的一半。结论大鼠阴茎包皮系带内含有浓密的nNOS免疫阳性神经末梢,这些神经末梢源自于L6-DRG和S1-DRG。Objective To study the distribution and origin of neuronal nitric oxide synthase （nNOS） immunoreactive nerve terminals in the prepuce and frenulum of penis in adult rats, Methods The nNOS-immunoreactive nerve terminals in the penile prepuce and frenulum were detected by the method of immunohistochemistry. The fluoro-gold （FG） retrogradely tracing and nNOS immunofluorescence was used to tracing the origin of nNOS-immunoreactive terminals in penile frenulum of rats. Results In the prepuce and frenulum of penis, dense nNOS-immunoreactive nerve terminals were mainly observed in the basal stratum of epidermis and papillary layer of dermis distributing in a cluster. These nerve terminals were arborescent and beady in appearance. The optical density of these nerve terminals is significantly higher in frenulum （3.4±0.38） than that in prepuce （2.2±0.45）. FG retrogradely labeled neurons were localized in L6-DRG （9.6±1.2）/mmeand SI-DRG （1.2±0.2）/mm^2 bilaterally, FG positive-neurons were arranged in rows or spots among nerve bundles. Further FG tracing method combined with nNOS indicated that a humble of DRG neurons were showed nNOS-immunoreactive（L6-DRG： 24.2±2.6）/mm^2, S 1-DRG： （24.1±2.1）/mm^2. 41.7% and 41.6% of FG-labeled neurons also have nNOS-immunoreactive expression in L6-DRG and S 1-DRG respectively. Conclusion There are a large number of nNOS-immunoreactive nerve terminals observed in the prepuce and frenulum of penis. These nerve terminals in the penile frenulum of rats were originated from peripheral branches of neurons which express nNOS-positive immunoreaction in L,-DRG and S1-DRG.

关 键 词：包皮系带 神经型一氧化氮合酶 荧光金 神经节 免疫组织化学 荧光免疫测定 

分 类 号：R339.21[医药卫生—人体生理学]