睡眠呼吸紊乱儿童腺样体中糖皮质激素受体的表达  

Glucocorticoid receptor subunit expression in adenoid tissue of children with obstructive sleep apnea bypopnea syndrome

作　　者：张孝文[1] 李源[1] 谢民强[1] 张革化[1] 刘贤[1] 王振霖[1] 王涛[1] 李仲汉[1] 

机构地区：[1]中山大学附属第三医院耳鼻咽喉头颈外科,广州510630

出　　处：《中华耳鼻咽喉头颈外科杂志》2007年第2期109-112,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

基　　金：国家教委博士基金(21030558080);中国博士后基金(20060390214)

摘　　要：目的了解阻塞性睡眠呼吸暂停低通气综合征（obstructive sleep apnea hypopnea syndrome，OSAHS）儿童腺样体组织中糖皮质激素受体（glucocorticoid receptor，GR）亚型mRNA表达。方法34例儿童行腺样体切除，术前睡眠监测；应用荧光定量逆转录PCR法检测患者腺样体组织中GR-d和GR-BmRNA的表达。结果腺样体组织中均存在GR-d和GR-8的mRNA表达，且GR-d mRNA呈高表达。OSAHS患儿（22例）腺样体组织中的GR-d和GR-B mRNA（x±s，以下同）分别为（9．404±3、06）×10^5cDNAcopies／μg和（1．574±0.3）×10^4cDNA copies／μg；非OSAHS患儿（12例）腺样体组织中的GR-α和GR-βmRNA分别为（1．604-0．26）×10^6 cDNA copies／μg和（1．524±0．18）×10^4cDNAcopies／μg。非OSAHS患儿腺样体组织中的GR-α mRNA较OSAHS患儿腺样体组织中的GR-α mRNA增高（F=40．285，P〈0．001）；OSAHS患儿腺样体组织中的GR-β mRNA较非OSAHS患儿腺样体组织中的GR-β mRNA稍高，但差异无统计学意义（F=0．145，P=0、706）。OSAHS、非OSAHS腺样体组织中GR-α／GR-β比值分别为62．34-20．3和107、44-24．4，差异有统计学意义（F=33．059，P〈0．001）。结论OSAHS儿童腺样体组织中存在糖皮质激素受体亚型表达，非OSAHS儿童腺样体对糖皮质激素可能比OSAHS儿童更敏感。GR在儿童OSAHS中的作用有待进一步研究。Objective The expression of glucocorticoid receptor （GR） expression in obstructive sleep apnea hypopnea syndrome （OSAHS） in children is currently unknown. The aim of this study was to determine the GR-α and GR-βstatus in the adenoidal tissues in children with OSAHS. Methods Thirty-four podiatric patients （aged 3-14 years, median 7.8 years ） had sleep study with polysomnography before adenoidectomy. According to the criteria of apnea hypopnea index （AHI） ≥5/h or/and apnea index （AI） ≥1/h, they were divided into OSAHS and non-OSAHS sub groups. The study was based on fluorescent quantitative PCR（FQ-RT-PCR）for the mRNA expression of GR-α and GR-β in the adenoidal tissues in children. Results GR isoforms mRNA encoding for expression of both GR-α and GR-β were detected in the adenoids of all children. GR-α mRNA level [ （9. 40 ±3.06） × 10^5 eDNA copies/μg total RNA] in the adenoidal tissues in OSAHS was lower than those in the non-OSAHS[ （ 1.60 ±0. 26） × 10^6 cDNA copies/μg total RNA ] （ F = 40. 285, P 〈 0. 001 ） , whereas no differences found for GR-β [ （ 1.57 ± 0. 35 ） ×^ 104 cDNA copies/μg total RNA, （ 1.52 ± 0. 18 ） × 10^4 cDNA copies/μg total RNA ]. GR-α/GR-β ratio was 62. 3 ± 20. 3 in OSAHS and 107.4 ±24. 4 in non-OSAHS. AHI or AI was not related to the mRNA levels of GR-α and GR-βin OSAHS or non-OSAHS. Conclusions GR-α and GR-β were detectable in the adenoidal tissues in children. These data indicated that the relationship between the expressions of GR and the clinical significance in OSAHS need further and profound investigation.

关 键 词：增殖腺 受体 糖皮质激素 RNA 信使 儿童 睡眠呼吸暂停 阻塞性 

分 类 号：R766[医药卫生—耳鼻咽喉科]