新生鼠窒息后心肌基质金属蛋白酶活性与心肌损害的相关性  

Correlation between matrix metalloproteinases activities and myocardial injury in neonatal rats after asphyxia

作　　者：徐书珍[1] 王彩霞[1] 赵炜[1] 初建芳[1] 刘文东[1] 李明[1] 

机构地区：[1]青岛市市立医院儿科,266011

出　　处：《中华儿科杂志》2007年第2期134-137,共4页Chinese Journal of Pediatrics

摘　　要：目的探讨新生鼠窒息后心肌组织心肌基质金属蛋白酶(MMPs)表达与心肌损害的相关性。方法 60只7～10日龄 Wistar 鼠,随机分成对照组(D 组)、窒息组(A、B、C 3组,分别为窒息复氧后1、7、14 d),每组15只。窒息组模拟新生鼠常压窒息模型,测定 cTnI 水平;心肌组织 MMPs-3、9活性;心肌病理积分及胶原含量。结果 cTnI(ng/ml)A 组(0.3680±0.40)明显高于 D 组(0.0783±0.06)P<0.05,B 组下降(0.1889±0.15)P 仍<0.05,C 组降至正常(0.1338±0.07)P>0.05;心肌组织 MMPs-3活性 A 组(0.1847±0.04),B 组最高(0.2780±0.05),C 组(0.2014±0.05),与 D 组(0.1213±0.03)比较 P 均<0.05;MMPs-9活性 A、B、C 组逐渐增高(0.1502±0.01、0.1715±0.01、0.1868±0.03、P 均<0.05);心肌胶原含量 A、B、C 组逐渐增高(35.59±1.09、38.94±0.67、40.69±0.75 ng/ml 分别为 P>0.05、P<0.05、P<0.05);MMPs-3、9表达与心肌病理积分呈正相关(r分别为0.669、0.667,P 均<0.05),与胶原含量呈正相关(r 分别为0.482、0.679,P 均<0.05)。结论新生鼠窒息后心肌 MMPs-3、9过度激活,继发胶原含量增高,MMPs-3、9活性与心肌病理损害密切相关。提示 MMPs 参与了窒息后心肌损害,可能是心肌间质重塑的因素之一。Objective To study possible correlation between matrix metalloproteinases （MMPs） activities and myocardial injury after asphyxia in neonatal Wistar rats. Method Sixty neonatal Wistar rats （7 to 10 days old） were randomly divided into four groups： control group （group D） ; asphyxia groups A,, B and C（1 day,7 days, 14 days after asphyxia）, every group had 15 rats. In the asphyxia groups, animal model was produced by normobaric asphyxia. Groups A, B and C were sacrificed on days 1, 7 and 14 days after asphyxia,and group D rats were sacrificed on the 7th day. Then the heart blood was taken to tested the serum cTnI. The myocardial MMPs-3and 9 activity was measured by using immunohistochemical assay. Histological sections of the hearts were stained with hematoxylin-eosin and myocardial histopathological scores were determined under an optical microscope. The amount of myocardial collagen was measured by means of chloramines T. Results cTnI was significantly higher in group A （0. 3680 ± 0. 40ng/ ml） than group D （0.0783±0.06 ng/ ml） （P 〈 0.05）,and was lower in group B（0.1889 ± 0.15ng/ ml） but still significantly different from that of group D （ P 〈 0. 05 ）, and declined to the normal level in group C （0. 1338 ± 0. 07ng/ ml）, but the difference between groups C and D was not significant （P 〉 0. 05 ）. Myocardial tissue MMPs-3 activity was transiently high in group A （0. 1847 ± 0. 04）, higher in group B （0.2780 ±0.05）as compared to group D（0. 1213 ±0.03） （P 〈0.05 for all）. The activity of MMPs-3 increased earlier than that of MMPs-9. The amount of myocardial collagen of group B （ 38.94 ± 0. 67 ） and C （40. 69 s0. 75） was significantly greater than that of group D （P 〈 0. 05）. Myoardial tissue MMPs-3 and MMPs-9 positively correlated with myocardial histopathological scores （ r =0. 669,0. 667, P 〈 0. 05） and myocardial collagen （r = 0. 482, 0. 679 , P 〈 0. 05）. Conclusions In rats with asphyxia, there was an excess activat

关 键 词：基质溶解素1 明胶酶B 胶原 肌钙蛋白I 窒息 新生儿 大鼠 

分 类 号：R722.1[医药卫生—儿科]