毛细管电泳-电致化学发光法测定口服液中磷酸苯丙哌林的含量  

Determination of Benproperine Phosphate in Oral Solution with Capillary Electrophoresis-Eelectrochemiluminescence

作　　者：彭志兵[1] 杨佳 汪敬武[1] 

机构地区：[1]南昌大学化学系,江西南昌330031 [2]特康科技股份有限公司,江西南昌330029

出　　处：《南昌大学学报（理科版）》2006年第6期552-555,共4页Journal of Nanchang University(Natural Science)

摘　　要：基于磷酸苯丙哌林增强三联吡啶钌的电致化学发光信号，建立了一种分离检测磷酸苯丙哌林的毛细管电泳一电致化学发光新方法。采用未涂层石英毛细管30cm×25μm；分离缓冲溶液为10mmo／L磷酸盐缓冲溶液（pH=8．0）；分离电压18kV；检测池中溶液为50mmo／L磷酸盐缓冲溶液（pH=8．0）和5mmo／L三联吡啶钌。在100s内可实现磷酸苯丙哌林的分离检测，其线性范围为（10^-7-10^-5）mol／L（相关系数0．9996），检出下限为10^-8mol／L。该法操作简便快速，灵敏度高，结果准确可靠，可用于该类药物的质量监测。A novel and sensitive method for the determination of benproperine phosphate in oral solution has been established using capillary electrophoresis （CE） coupled with electrogenerated- chemiluminescence（ECL） detection,based on the ECL reaction of tris（2,2＇-bipyridyl） ruthenium（ Ⅱ ） with the tertiary amino groups of the benproperine analyte. Electrophoresis was performed using an uncoated silica capillary （30 cm × 25 μm）. 10 mM sodium phosphate buffer （pH = 8.0） was used as the running buffer and the separation voltage was 18 kV. The solution in the detection cell was 50 mM sodium phosphate （pH = 8. 0） and 5 mM Ru （bpy）3^2＋. Benproperine phosphate in the sample could be separated and detected with in 100 s. The linear concentration of benproperine phosphate ranged from 10 7 to 10 5 mol/L （with a correlation coefficient of 0. 999 6）. The limit of detection was 10 s mol/L （S/N =3）. This method is simple and quick in operation,sensitive and reliable in determination results and it can be used for the quality control of benproperine phosphate.

关 键 词：磷酸苯丙哌林 毛细管电泳 电致化学发光 三联吡啶钌 

分 类 号：O657.8[理学—分析化学]