高效液相色谱法测定稻·噁乳油含量  

Determination of Active Ingredients in Mixture Formulation Kiazine-oxamyl Emusifiable Concentrate by HPLC

作　　者：刘玮玮[1] 慕卫[1] 朱炳煜[1] 魏光[1] 翟茹环[1] 刘跃群[1] 

机构地区：[1]山东农业大学植保学院,山东泰安271018

出　　处：《精细化工中间体》2007年第1期67-68,72,共3页Fine Chemical Intermediates

基　　金："十五"国家科技攻关计划资助项目(2001BA509B08);山东农业大学博士后基金资助项目

摘　　要：采用高效液相色谱法,在同一色谱条件下对稻·噁乳油中所含有效成分稻瘟灵和噁霉灵同时进行含量测定。色谱条件为:使用Nova-PaK C18250mm×4.6mm(i.d)不锈钢色谱柱,流动相v(甲醇)∶v(水)=70∶30,流速1mL/min,检测波长为220nm。测定方法中稻瘟灵和噁霉灵的回收率分别在98.8%～100.3%和98.6%～100.0%之间,变异系数分别为0.54%和0.52%,证明本方法快速、准确、可靠。The content of kiazine and oxamyl in the formulation kiazine-oxamyl emusifiable concentrate was determined by HPLC in the same chromatogram conditions. The operating conditions by HPLC were C18 column （250 min ×4.6 mm）, v（methanol） ： v （water） =70 ： 30 as mobile phase, 1 mL/min as flow speed and wavelength 220 nm. The recoveries of kiazine and oxamyl were 98.8%-100.3% and 98.6 %-100.0%, respectively, and their coefficients of variation were 0.54% and 0.52%, respectively, indicating that this method is fast, accurate and repeatable.

关 键 词：稻瘟灵 噁霉灵 HPLC 

分 类 号：S482.299[农业科学—农药学]