柠条锦鸡儿的组织培养  

Study on the Tissue Culture of Horqin Peashrub

作　　者：宋俊双[1] 王赞[1] 孙桂芝[1] 高洪文[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094

出　　处：《草地学报》2007年第1期66-69,共4页Acta Agrestia Sinica

基　　金：国家"863"项目(2002AA241091)

摘　　要：以柠条锦鸡儿(C arag ana korsh insk ii K om.)无菌苗的子叶和下胚轴为外植体,研究其愈伤组织诱导及植株再生。结果表明:柠条锦鸡儿子叶和下胚轴均能诱导出愈伤组织,其适宜诱导培养基分别为M S+2,4-D 2.0(m g/L)+KT 0.1(m g/L)和M S+2,4-D 2.0(m g/L)+BA 0.5(m g/L);子叶诱导不定芽的适宜培养基为M S+6-BA 0.2(m g/L)+NAA 0.05(m g/L),下胚轴诱导不定芽的适宜培养基为M S+6-BA 0.5(m g/L)+NAA 0.05(m g/L);适宜生根培养基为1/2M S+IBA 0.5(m g/L)+NAA 0.5(m g/L)。本试验结果可为柠条锦鸡儿的快速繁殖利用及品种改良提供依据。A study on callus induction and plantlet regeneration of Horqin peashrub （Caragana korshinskii Kom. ） was carried out. The results show that both the cotyledon and hypocotyl of the plant could successfully induce callus, for which the optimum media were MS＋2,＋D 2.0（mg/L）＋KT 0. 1（mg/L）, and MS＋2,＋D 2.0 （mg/L）＋BA 0. 5 （mg/L）, respectively. The best media for inducing adventitious buds by cotyledon and hypocotyl were MS＋ 6-BA 0. 2（mg/L）＋NAA 0. 05（mg/L）, and MS＋ 6-BA 0.5 （mg/L） ＋ NAA 0.05 （mg/L）, respectively, while the optimum rooting medium was 1/2MS＋IBA 0. 5 （mg/L） ＋ NAA 0. 5（mg/L）. Some suggestions for the rapid propagation and variety improvement of Caragana korshinskii are offered in this study.

关 键 词：柠条锦鸡儿 组织培养 外殖体 激素浓度 

分 类 号：Q943[生物学—植物学]