新生鼠缺氧缺血性脑病水通道蛋白4与肿瘤坏死因子-α的变化及意义  

Changes of aquaporion-4(AQP4)and TNF-α in the neonatal rats with hypoxic-ischemic encephalopathy

作　　者：张费通[1] 崔其亮[1] 刘海燕[1] 黎刚[2] 谭岱峰[1] 

机构地区：[1]广州医学院第三附属医院儿科,510150 [2]广州医学院第三附属医院药剂科,510150

出　　处：《实用医学杂志》2007年第3期339-341,共3页The Journal of Practical Medicine

基　　金：广东省医学科学技术研究基金资助项目(编号:A2006526)

摘　　要：目的:研究新生鼠缺氧缺血性脑病后大脑中水通道蛋白4(AQP4)与肿瘤坏死因子-α(TNF-α)的变化及意义。方法:35只7日龄新生SD大鼠分为7组,包括对照组(C组)和实验组6组(缺氧缺血组),分别在缺氧缺血性脑病0、2、4、6、8、12h断头取脑,做病理切片,免疫组化分析,脑组织匀浆用放免法测定细胞因子TNF-α。结果:AQP4主要表达在脉络丛、室管膜上皮、海马及水肿区,随着时间的延长,AQP4表达呈增高趋势。对应的病理改变为细胞内水肿、炎症反应。脑组织内细胞因子TNF-α在缺氧缺血性脑病2h与对照组相比即明显增加(P<0.05)。结论:AQP4和TNF-α与新生鼠缺氧缺血后的脑水肿关系密切,且二者之间可能有某种关系,抑制AQP4和TNF-α可为治疗新生儿缺氧缺血性脑病开辟新的途径。Objective To observe the changes of AQP4 expression and TNF-α in the neonatal rats with hypoxic-ischemic encephalopathy （HIE）. Methods Seven-day-old neonatal rats were devided into seven groups, including control group（n=5） and operation group（n=30）, The operation group was executed after HIE at 0,2,4,6,8 and 12 h, respectively. Its brain issue was collected to quantify the changes in the area of the AQP4-pesitive cell by immunocytochemistry analysis. TNF-α levels were detected by radioimmunological methods. Results The AQP4 expression was predominantly observed in choroids plexus, ependyma, hippocampus and edema area. With the progress of the time, the expression of AQP4 increased slowly. The pathologic changes of the stage were intracellular inflammatory reaction. Contents of TNF-α were significantly increased 2 h in operation group than control group （P 〈 0.05 ）.Conclusions AQP4 and TNF-α are closely related with the development of brain edema after HIE. So, inhibition of AOP4 and TNF-α will be a new way to treat HIE.

关 键 词：脑缺血 缺氧 脑 脑水肿 水孔蛋白类 

分 类 号：R722.1[医药卫生—儿科]