狗牙根品种及辐射诱变M_1代变异植株的过氧化物同工酶分析  

Analysis on Peroxidase Isozyme of the Cynodon dactylon Cultivars and Mutants Induced by Irradiation

作　　者：王文恩[1] 包满珠[1] 张俊卫[1] 

机构地区：[1]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《中国草地学报》2007年第1期71-76,共6页Chinese Journal of Grassland

基　　金：国家"863"计划项目(2004AA244050)

摘　　要：采用聚丙烯酰胺凝胶电泳法对辐射诱变M1代变异植株及其对照、‘Tifway’和‘Tifgreen’共18个狗牙根材料的过氧化物同工酶进行了分析。结果表明:供试材料共出现了97条酶带,单个材料最多为8条,最少只有2条;材料HN009和HN010、HN012和HN013的POD酶带特征完全相同;HN015和HN016的POD酶带在P8位点上有强弱不同,HN004和HN018的POD酶带在P5和P6位点上有强弱不同,它们的酶带数和位点均相同;其它各材料之间酶带总数不同,在各个区域分布的位点及酶谱特征明显不同。除HN010和其对照HN009的酶谱特征相同外,其它形态变异植株和其对照之间的酶谱特征均存在明显差异。The POD isozyme of 18 accessions （including mutants, control, ‘Tifway＇ and ‘Tifgreen＇）of Cynodon dactylon were analyzed by polyacrylamide electrophoresis. Totally 97 isozyme bands were obtained, the maximum was 8 and the minimum was 2 for individual accession the band pattern was identical between HN009 and HN010, HN012 and HN013; The number of bands and locus was identical between HN015 and HN016 except for the difference of strength in P8 locus, and so was the case between HN004 and HN018 except for the difference on P5 and P6 locus strength The number of bands and locus were ob viously different among the other materials. The difference of band pattern were obvious between mutants and the control plant except for mutant HN010.

关 键 词：狗牙根 辐射诱变 变异植株 过氧化物同工酶 

分 类 号：S543.9[农业科学—作物学]