急性细菌性腹膜炎对豚鼠小肠Cajal间质细胞的影响  

Effects of acute bacterial peritonitis on interstitial cells of Cajal in intestinal wall of the guinea pigs

作　　者：龚斌 梅龙村 

机构地区：[1]重庆市渝北区第二人民医院,重庆401147

出　　处：《局解手术学杂志》2007年第1期19-21,共3页Journal of Regional Anatomy and Operative Surgery

摘　　要：目的 探讨豚鼠腹膜炎模型中小肠Cajal间质细胞网络的变化情况。方法 利用腹腔内注射大肠杆菌和10％BaSO4肉汤佐剂的方法建立腹膜炎模型，冰冻切片和全层铺片并结合KIT免疫细胞化学染色观察。结果 腹膜炎12～24h，腹膜炎症状最明显，在切片和铺片上均可见ICCs明显减少，其中IC-MY减少最显著，48～96h后ICCs的数量逐渐恢复正常，同时豚鼠的腹膜炎反应也减轻甚至康复。结论 小肠壁内ICCs在腹膜炎模型中可明显减少，但随着时间延长，ICCs逐渐恢复正常的细胞网络。Objectives To investigate the changes of interstitial cells of Cajal networks in the guinea pigs model of acute bacterial peritonitis. Methods Peritonitis models were carried out by injection of bacterium coli and 10% BaSO4 broth adjuvant, ICCs were observed on the frozen sections and whole-mount preparation with KIT immunohistochemistry. Results 12 - 24 hours after peritonitis, symptoms of peritonitis were observed and ICCs decreased obviously on both frozen sections and whole-mount preparation, especially for IC-MY, while 2 - 4 days later the number and distribution of ICCs restored to normal. Conclusion Acute bacterial peritonitis might lead to temporary loss of ICCs, and it would be soon recovered.

关 键 词：CAJAL间质细胞 KIT蛋白 小肠 腹膜炎 

分 类 号：R656.41[医药卫生—外科学] R361.2[医药卫生—临床医学]