心经经脉与心脏相关的差异表达基因的研究  

Research on the Differentially Expressed Genes Related to the Relatively Specific Connection Between the Heart Meridian and the Heart

作　　者：周逸平[1] 汪克明[1] 胡玲[1] 吴子建[1] 周美启[1] 王月兰[1] 杨黄恬[2] 张发宝[2] 孙露 

机构地区：[1]安徽中医学院经脉脏腑相关研究中心,合肥230038 [2]中国科学院上海生命科学研究院健康科学中心心脏分子学实验室,上海200025 [3]上海晶泰生物科学技术有限公司,上海200223

出　　处：《针刺研究》2007年第1期3-8,共6页Acupuncture Research

基　　金：国家重大基础前期研究专项(2002CCD02500);"973计划"(2005CB523306-2;2005CB523308)

摘　　要：目的:从基因差异表达方面探讨心经与心脏相关的特异性基础。方法:复制大鼠急性心肌缺血模型,分别电针心经“神门”至“通里”段、小肠经“养老”至“支正”段、肺经“太渊”至“列缺”段。刺激电压5V,频率2Hz,波宽300μs,正负向交替方波,每次电刺激20min,1次/d,共3次。取缺血心肌组织,采用大鼠全基因U230序列芯片进行芯片检测,大规模筛选差异表达基因。结果:与模型组比较,差异表达2倍以上的基因,电针心经组有20个上调70个下调;电针小肠经组有18个上调26个下调;电针肺经组有14个上调20个下调,且3组之间鲜有相同的基因表达。结论:经脉脏腑相关具有确实的分子基础,电针心经、小肠经、肺经具有相对特异性作用途径。Objective： To study the scientific basis of specific correlation between the meridian and the Zeng-or Fu-organ by using gene differential expression （gene-chip technique） in the rat. Methods： Twenty-one SD rats were randomly and evenly divided into control group, sham-myocardial ischemia （MI） group, MI group, electroacupuncture （EA）-Heart-Meridian （HT） group, EA-Lung-Meridian （LU） group and EA-Small-lntestine-Meridian （SI） group. EA （frequency 2 Hz, stimulating strength 5 V, pulse duration 300 μs） was applied to ＂Shenmen＂ （HT 7）- ＂Tongli＂ （HT 5） （Heart Meridian）, ＂Yanglao＂ （SI 6）- ＂Zhizheng＂ （SI 7） （Small Intestine Meridian）, ＂Taiyuan＂ （LU 9）- ＂Lieque＂ （LU 7） （Lung Meridian） for 20 min, once daily and continuously for 3 days. Myocardial ischemia model was duplicated by occlusion of the anterior descending branch of the left coronary artery, and myocardial tissue of the left ventricle was taken 3 days after EA treatment for analyzing the differentially expressed genes in different groups with rat whole gene U 230 sequence chips （offered by Affymetrix Company）. Results： Compared with model group, among the differentially expressed genes being twofolds and above, the up-regulated and the down-regulated were 20 and 70 in EA-HT group, 18 and 26 in EA-SI group, and 14 and 20 in EA-LU group separately. Few same genes with the same differential expression were found among the 3 EA groups. Conclusion： There is exactly a molecular basis in the correlation between Heart Meridian and the heart, and EA of Heart Meridian, Small Intestine Meridian and Lung Meridian have their own special signaling pathways.

关 键 词：经脉脏腑相关 基因芯片 差异表达基因 心肌缺血 电针 

分 类 号：Q343.17[生物学—遗传学]