穴区肥大细胞功能与针刺缓解急性佐剂性关节炎大鼠疼痛效应的关系  

Relationship Between the Function of Mast Cells and Acupuncture Analgesia in Adjuvant Arthritis Rats

作　　者：林俊[1] 黄红[1] 丁光宏[1] 张迪[1] 

机构地区：[1]复旦大学力学与工程科学系,上海200433

出　　处：《针刺研究》2007年第1期16-19,共4页Acupuncture Research

基　　金：973项目(2005CB523306);上海市科技发展基金;上海市教委重点学科项目(T0302)

摘　　要：目的:通过观察针刺镇痛效应与穴位区肥大细胞脱颗粒率的变化,研究针刺效应与穴位局部的肥大细胞功能之间的关系。方法:58只SD雄性大鼠随机分为正常组、模型组、留针组(仅留置针体在穴位处)、电针(EA,2/100Hz,0.5/1.0/1.5mA,“足三里”)组和手针(MA)组,在急性佐剂性关节炎(AA,完全弗氏佐剂0.05mL注射于动物踝关节)大鼠模型上,采用辐射热缩爪反射的方法,以大鼠缩爪反射的潜伏期为观察指标,比较电针、手针和留针组的镇痛效果。将穴位区的组织块制成石蜡切片,用甲苯胺蓝特殊染色法染色,观察其中肥大细胞变化的情况。结果:电针和手针对AA大鼠都有很明显的镇痛效应(与留针组比较P<0.01),而留针组无效(与模型组比较P>0.05),其中手针穴位处的效果更明显于电针。同时,电针和手针组的组织切片中肥大细胞脱颗粒率明显高于留针组(P<0.01)。结论:穴位组织中的肥大细胞在针刺镇痛效应的产生过程中起着重要作用,肥大细胞脱颗粒参与了针刺镇痛效应中针刺信号从穴位向靶器官传输与转换的过程。Objective： To investigate the relationship between mast cell degranulation and acupuncture analgesia, Methods： A total of 58 SD male rats were randomly divided into control （n = 12）, model （n = 12）, needle-retaining （n = 12）, electroacupuncture （EA, n=12） and manual-acupuncture （MA, n=10） groups, Arthritis model was established by injection of complete Freund＇s adjuvant （0.05 mL） into the left ankle-joint. The paw withdrawal latency （PWL） was detected with a radiant heat algesimeter. Electroacupuncture （EA, 2/100 Hz, 0.5-1.5 mA） or manual acupuncture （lifting and thrusting force being 240-280 mN, torque being 10- 15 mN · mm, 3 times/s） were applied to ＂Zusanli＂ （ST 36） on the affected side for 30 min in the corresponding group. After decapitation （under anesthesia）, skin tissue around the stimulated ST36 was sampled, cut into sections （5 μm）, and stained with Toluidine Blue for observing the count of degranulated mast cells. Results： In comparison with control group, PWL values of model, needle-retaining, EA and MA groups decreased significantly after modeling （P〈0.01）; while compared with needle-retaining group, PWL values of EA and MA group increased considerably （P 〈0.01 ）, suggesting production of analgesia, After EA and MA stimulation, degranulation of mast cells in the stimulated ST36 area appeared apparently. Compared with needle-retaining group, the degranulation ratios of mast cells in EA and MA groups were obviously higher （P〈 0.01）. Conclusion： Both EA and MA stimulation of ＂Zusanli＂ （ST 36） can effectively raise pain threshold, simultaneously, and enhance the degranulation ratio of mast cells in the stimulated ST36 area.

关 键 词：穴位 肥大细胞 脱颗粒率 针刺镇痛 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]