囊素对猪口蹄疫灭活疫苗的免疫增强作用  

Enhancement of bursin on inactivated foot-and-mouth disease vaccine in piglets

作　　者：朱志盈[1] 缪德年[2] 姜法铭[2] 曹海军[1] 姚惠娟[2] 潘饶霖[2] 赵本进[2] 樊生超[2] 陆承平[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《中国兽医科学》2007年第2期150-154,共5页Chinese Veterinary Science

基　　金：上海市农业"四新"技术推广项目[沪农科推字(2003)第3-2-2]

摘　　要：用提取的天然囊素（10ug／mL）和O型口蹄疫灭活疫苗协同免疫45日龄仔猪，研究了其对。型口蹄疫灭活疫苗免疫效果及仔猪增重的影响。将32头健康45日龄仔猪随机分为4组，0．5mL疫苗＋囊素免疫2次（Ⅰ）组、1、0mL疫苗＋囊素免疫2次（Ⅱ）组、1．0mL疫苗＋囊素免疫1次（Ⅲ）组和1．0mL疫苗免疫2次（Ⅳ）组（疫苗对照组），用液相阻断酶联免疫吸附试验（LB—ELISA）检测各组猪免疫前及一免后第14、28、42、60、74、88、102d的口蹄疫血清抗体效价并称重。结果。Ⅱ组抗体水平显著高于Ⅳ组（P〈0．01），Ⅰ组、Ⅲ组和Ⅳ组间差异不显著（P〉0．05）；一免后第60～110d仔猪平均日增重囊素组明显高于对照组（P〈0．05）。结果表明，囊素可以提高口蹄疫灭活疫苗的免疫原性，提高猪增重，促进生长发育。To evaluate the effects of the inactivated foot and-mouth disease（FMD） vaccine co-administrated with the extracted natural bursin（10 μg/mL） on level of the antibody against foot-and-mouth disease virus（FMDV） and weight of piglets, thirty-two 45 day-old healthy piglets were randomly divided into groups Ⅰ , Ⅱ , Ⅲ and Ⅳ （control）, and were injected with the inactivated FMD vaccine when they were 45- day-old and 59-day-old, and injected with 4 and 5 mL of bursin when they were 45-and 105 day-old, respectively. The titersof the antibody against FMDV were detected by LB-ELISA on days 0,14,28,42,60,74, 88 and 102 post inoculation and the piglets＇ weights were measured. The results showed that the serum an- tibody against FMDV of Group Ⅱ （1.0 mL of vaccine co-administrated with bursin for twice） was significantly higher than that of the control group（P〈0. 01）, whereas there were no significant difference among Group Ⅰ （0.5 mL of vaccine co-administrated with bursin for twice）, Ⅲ （1.0 mL of vaccine co-administrated with bursin for once）and control group（P〈0. 05）. The average daily weight-gain was higher in bursin group than the control group from day 60 to day 110 post-inoculation（P〈0.05）. It was concluded that the bursin could enhance the immunogenicity of the vaccine and the growth of the inoculated piglets.

关 键 词：囊素 口蹄疫灭活疫苗 佐剂 增重 

分 类 号：S852.4[农业科学—基础兽医学]