流感病毒在不同细胞中适宜培养条件的研究  

Research on optimal culture condition for influenza virus in different cells

作　　者：范东瀛[1] 陈俊英[1] 张新文[1] 李卫东[1] 廖国阳[1] 

机构地区：[1]中国医学科学院中国协和医科大学医学生物学研究所,昆明650118

出　　处：《第三军医大学学报》2007年第6期510-512,共3页Journal of Third Military Medical University

基　　金：云南省自然科学基金面上项目(200420070M);2003年度云南省科技厅创新人才引培项目(2003PY07)~~

摘　　要：目的探索流感病毒在细胞中培养的适宜条件。方法采用MEK细胞、FRhK-4细胞、MDCK细胞分别接种甲型流感病毒H1N1型、H3N2型和乙型流感病毒疫苗毒株,观察3种毒株在不同培养条件下的细胞病变、血凝滴度。结果3株流感病毒接种细胞在33℃培养72h后出现拉网、圆缩、脱落等现象;FRhK-4细胞和MDCK细胞对流感病毒较敏感。胞龄为48h的细胞,在NaHCO3的终浓度为1.4‰,TPCK-胰酶浓度为1.0μg/ml的病毒维持液培养流感病毒为最适条件。结论寻找到了细胞流感病毒的适宜条件。Objective To search for optimal culture condition of influenza virus in the tender cells. Methods Influenza A （H3N2, H1N1） and B virus were inoculated in MDCK cells, MEK cells and FRhK-4 cells under different culture conditions to observe cytopathic effect （CPE） , hemagglutination titer. Results after incubated at 33℃ for 72 h, cytopathic effect of viral infection in all three kinds of cells was observed, and hemagglutination assay showed MDCK cells and FRhK-4 cells were more sensitive than MEK cells to influenza vi- rus. It is the best culture condition that the cells aged 48 h in maintenance media containing 1.4‰ NaHCO3, 1.0 μg/ml trypsin-TPCK. Conclusion The optimal culture conditions for influenza virus in cells were showed in this study.

关 键 词：流感病毒 细胞培养 血凝滴度 

分 类 号：Q939.4[生物学—微生物学] R373.13[医药卫生—病原生物学]