水红花子醇提物抑制大鼠组织脂质过氧化反应的体外作用研究  

Effect of alcoholic extract from Fructus Polygoni Orientalis on lipid peroxidation in rat tissues

作　　者：葛斌[1] 张振明[1] 许爱霞[1] 高湘[1] 雷晓燕[2] 

机构地区：[1]甘肃省人民医院药剂科,兰州730000 [2]甘肃省人民医院小儿科,兰州730000

出　　处：《第三军医大学学报》2007年第6期516-518,共3页Journal of Third Military Medical University

基　　金：甘肃省自然科学基金计划项目(3ZS041A25073)~~

摘　　要：目的研究水红花子醇提物的抗氧化活性。方法用·OH生成系统Fe2++抗坏血酸诱导大鼠心、肝、肾组织匀浆脂质过氧化,用TBA比色法测定MDA含量;用NBT还原法测酵母多糖A刺激大鼠中性白细胞产生的O2-;用分光光度法测H2O2诱发的大鼠红细胞溶血度。结果6.3、12.5、25、50、100、200μg/LEFPO能不同程度抑制Fe2++抗坏血酸诱导的大鼠心、肝、肾脂质过氧化产物MDA生成,其抑制心、肝、肾MDA生成的IC50分别为31.8、32.5、40.2μg/L,量效关系均呈负相关,r分别为-0.886、-0.874及-0.918(P<0·01);能不同程度抑制酵母多糖A刺激中性粒细胞生成O2-,IC50为31.9μg/L,量效关系呈负相关,r为-0.873(P<0·01);能不同程度抑制H2O2诱发的红细胞氧化溶血,IC50为56.2μg/L,量效关系呈负相关,r为-0.888(P<0·01)。结论水红花子醇提物通过清除·OH、O2-及H2O2发挥抗氧化活性。Objective To study the activities of the alcoholic extract from Fructus Polygoni Orientalis （EFPO） against antioxidation. Methods The peroxidation in homogenate of rat heart, liver and kidney was induced by · OH generation system Fe^2＋ ＋ ascorbic acid. Malondialdehyde （MDA） was determined by TBA colorimetric method. Superoxide （ O2^- ） from zymosan-stimulated neutrophils of rat was measured by NBT reduction method. H2O2-caused hemolysis of erythrocytes was measured by spectrometry. Results MDA in homogenate of heart, liver and kidney of rat that induced by · OH generation system Fe^2＋ ＋ ascorbic acid was inhibited by 6.3, 12.5, 25,50, 100,200 μg/L EFPO; IC50 of EFPO to MDA in heart, liver and kidney was 31.8, 32.5 and 40.2 μg/L respectively and its dose-effect relation was of negative relativity and the relative coeffcient was -0. 886, -0. 874 and -0. 918 （ all P 〈0.01 ）. 02- generation from zymosan-stimulated neutrophils of rat was inhibited by EFPO; IC50 of EFPO to O2^- generation was 31.9 μg/L and its dose-effect relation was of negative relativity and the relative coefficient was -0.873 （P 〈 0. 01 ）. H2O2-caused hemolysis of erythrocytes was inhibited by EFPO. ICs0 of EFPO to erythrocyte hemolysis was 56.2 μg/L and its dose-effect relation was of negative relativity and the relative coefficient was -0.888 （ P 〈 0. 01 ）. Conclusion EFPO exerts its antioxidation by scavenging · OH, O2 and H2O2 in biological system.

关 键 词：水红花子醇提物 丙二醛 自由基 溶血度 红细胞 中性粒细胞 抗氧化活性 

分 类 号：R282.71[医药卫生—中药学] R285.5[医药卫生—中医学]