老化对卵母细胞孤雌发育和减数分裂期间微管动态变化的影响  

The effect of aging on oocyte parthenogenetic development and the dynamic changes of microtubule during meiotic division

作　　者：冯秀清[1] 林英巍 钟淑琦[1] 陈雅隽[1] 阎晓飞[1] 董建将[1] 王琪[1] 雷蕾[1] 

机构地区：[1]哈尔滨医科大学组胚学教研室,黑龙江哈尔滨150081 [2]山东省东营市垦利县妇幼保健院,山东东营257500

出　　处：《哈尔滨医科大学学报》2007年第1期1-3,7,F0003,共5页Journal of Harbin Medical University

基　　金：国家自然科学基金资助项目(30671025);黑龙江省教育厅海外学人科研资助项目(1151hz031);黑龙江省卫生厅一般资助项目(2006-271)

摘　　要：目的研究老化对卵母细胞孤雌激活发育率的影响,以及老化对减数分裂期间微管的动态变化的影响。方法用孤雌激活的方法来观察不同老化时间胚胎的发育情况;用免疫荧光化学与激光共聚焦显微术结合的方法研究小鼠卵母细胞微管的动态变化。结果从注射hCG后14h到18h,卵母细胞在体外老化过程中激活率和发育率呈上升趋势,但是差异不显著。体外老化至18h,卵母细胞更容易被激活,并且减数分裂期间微管的变化发生要早于14h。结论短时间的体外老化可以使细胞更容易被激活,且微管发育更完善。Objective To detect the effects of aging on parthenogenetic development and dynamic changes of microbule during meiotic division in mice. Methods Artificial activation was utilized to address the developmental potential of ooeytes at deferent aging time. Immunofluoreacence and confoeal were employed to study the dynamic changes of microubulet in mouse ooeytes. Results The tendencies of developmental potentials and activation rates were raised from 14h to 18h after hCG injection. However, there had no significant difference between these.groups. Comparing with cells at 14h after hCG injeetion, cells at 18h after hCG injection was easier to activate the oocyte and the changes of the microtubule during meiotic division were earlier. Conclusion Short time of in vitro aging is beneficial to oocyte activation and microtubule development.

关 键 词：孤雌激活 老化 微管 卵母细胞 激光共聚焦 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R394.2[医药卫生—基础医学]