HPV6b L1重组蛋白在大肠埃希菌中的表达与鉴定  

Expression and identification of HPV6b L1 recombinant protein in E.coli

作　　者：韩聪[1] 王燕[1] 商庆龙[1] 魏兰兰[1] 刘希君[1] 谷鸿喜[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2007年第1期11-12,15,共3页Journal of Harbin Medical University

基　　金：哈尔滨市科技攻关计划项目(2004AA3cs176-1)

摘　　要：目的研究人乳头瘤病毒6b型主要衣壳蛋白L1(HPV6b L1)在原核系统中的表达及鉴定。方法酶切pBluescript M13-HPV6b重组质粒,构建原核表达质粒pBAD-HPV6b L1,并转入大肠埃希菌Top10菌株,经L-Arabinose诱导,表达融合蛋白6×His-L1,用SDS-PAGE和蛋白印迹方法进行检测。结果HPV6b L1蛋白在Top10菌中高效表达,其相对分子量60kD,并保有HPV6b L1特异性。结论HPV6b L1蛋白在原核表达系统中获高效表达,为该蛋白质的功能及HPV的基因工程疫苗的研究提供了物质基础。Objective To study the expression of HPV6b capsid proteins L1 in E. coli. Methods The HPV6b L1 gene was obtained from pBluescript M13-HPV6b by restricted digestion. The plasmid pBAD- HPV6bL1 was constructed by inserting the HPV6b L1 into pBAD and transformed into E. coli strain Top10. The recombinant strain was induced by L-Arabinose. SDS-PAGE and Westem blot were used to confirm the characteristics of the expressed proteins. Results A 60kD band could be demonstrated by SDS-PAGE. The specificity of HPV6b L1 had been verified by Westem blot. Conclusion Fusion protein 6×His-HPV6bL1 was expressed efficiently in E. coli Top10. This study provided possibility for evaluating the function of HPV6b L1 protein and developing HPV vaccine against condyloma accuminatum.

关 键 词：人乳头瘤病毒6b型 L1蛋白 尖锐湿疣 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]