新生猪缺氧后心肌连接蛋白Cx43表达的变化  

Expression of Cx43 in the Myocardia of Hypoxic Newborn Piglets

作　　者：夏红萍[1] 黄国英[1] 孙波[1] 陈超[1] 朱列伟[1] 

机构地区：[1]复旦大学附属儿科医院心血管中心,上海200032

出　　处：《中国实验动物学报》2007年第1期8-10,I0001,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：教育部高等学校骨干教师资助计划(编号:2000-66)

摘　　要：目的探讨新生猪缺氧后心肌连接蛋白Cx43 mRNA和蛋白含量的变化。方法对照组(C组):新生猪6头,未吸入低氧;缺氧组(H组):新生猪6头,吸入低氧(FiO2=0.1)维持1 h。于缺氧结束后5 h,免疫荧光染色检测心肌组织细胞缝隙连接蛋白Cx43蛋白的表达,实时荧光定量逆转录多聚酶链式反应(RT-PCR)检测心肌组织Cx43 mRNA的表达。结果H组心肌组织Cx43蛋白的表达显著低于C组(P<0.01),但两组心肌组织Cx43 mRNA表达差异无统计学意义(P>0.05)。结论缺氧可导致心肌Cx43蛋白的表达或分布发生变化。Objective To observe the protein and mRNA expression of Cx43 in the heart of hypoxic newborn piglets. Methods Twelve anesthetized and mechanically ventilated piglets （5 - 7 days old） were randomly divided into two groups： （1） Group C, not exposed to hypoxia; （2） Group H, inhaled 10% oxygen for 1 h. Five hours after hypoxia, Cx43 expression was revealed by immuno fluorescence staining. Cx43 mRNA was calculated by real-time fluorescence quantitative RT-PCR. Results After hypoxia, protein expression of Cx43 in group H was significantly decreased as compared with that of group C （ P 〈 0.01 ）. However, there was no significant difference in the mRNA expression of Cx43 between the two groups （ P 〉 0.05）. Conclusions Hypoxia may induce changes of the protein expression or location rather than the transcription of Cx43 in newborn piglets.

关 键 词：缺氧 新生猪 心脏 连接蛋白43 

分 类 号：R329.411[医药卫生—人体解剖和组织胚胎学]