丹黄方对硫代乙酰胺所致大鼠急性肝衰竭的防治作用  

Prevention and cure effect of Danhuangfang on acute hepatic failure rats thioacetamide-induced

作　　者：朱清静[1] 陈锋[2] 盛国光[1] 

机构地区：[1]湖北省中医院国家肝病治疗中心,湖北武汉430061 [2]湖北中医学院

出　　处：《中西医结合肝病杂志》2007年第1期35-37,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：湖北省自然科学基金课题资助(No.2004ABA236);湖北省教育厅重点科研项目(No.A04006)

摘　　要：目的:探讨丹黄方抗大鼠急性肝衰竭的作用和可能机制。方法:按600mg/kg的剂量经皮下注射硫代乙酰胺(TAA)1次,24小时后按相同剂量重复注射1次,造成大鼠急性肝衰竭模型,于实验前3天至实验结束分别给予各组大鼠生理盐水,不同剂量丹黄方及促肝细胞生长素,观察造模后48小时内大鼠病死率,并于造模48小时后经腹主动脉采血测定ALT、AST、TBil、TNF-α、IL-6,取肝组织作病检。结果:丹黄方能明显降低急性肝衰竭大鼠死亡率、降低ALT、AST、TBil及TNF-α、IL-6水平,改善肝组织病变,与模型组及促进肝细胞生长素阳性对照组比较,P<0.05或P<0.01,差异有显著性意义。结论:丹黄方具有抗TAA大鼠急性肝衰竭作用,可能与降低大鼠急性肝衰竭的重要介质TFN-α、IL-6有关。Objective： To explore the effects and probable mechanism of Danhuangfang （DHF） on rats with laboratory acute hepatic failure. Methods： The rats were injected with thioacetamide （TAA） 600mg/kg twice at same time for two days to induce the model of acute hepatic failure rat except the normal group injected with the normal saline hypodermically. The rats were treated with the normal saline, DHF low, middle and high dosages, PHGF respectively every day for 3 days before TAA were injected . To observe the mortality of the rats in 48 hours, and 48 hours later, the blood were collected to measure the indexes of ALT, AST, TBil, tumor necrosis factor （TNF-α）, interleukin-6 （IL-6） , and at the same time, pathological morphologies of hepatic tissues were observed respectively. Results： The DHF treatment group was super to controlled group in the mortality and the indexes reducing ALT, AST, TBil, TNF-α, IL-6, improving liver tissue pathological changes of rats with acute hepatic failure. Comparing with model group and control group, there are significant difference （P 〈0. 05 or P 〈 0. 01 ）. Conclusion： The DHF have resistance against the rat with acute hepatic failure by TAA, it related to decrease TNF-α the important medium of acute hepatic failure and IL-6.

关 键 词：丹黄方/药效学 硫代乙酰胺 急性肝衰竭 肿瘤坏死因子-Α 白介素-6 

分 类 号：R285.5[医药卫生—中药学]