基于有机-无机杂化材料和铂电极的葡萄糖氧化酶电极的研究  

Amperometric Glucose Biosensors Based on Platinum Electrode and Organic-Inorganic Hybrid Material

作　　者：于洋[1] 赵林[1] 闫博[1] 谭欣[1] 

机构地区：[1]天津大学环境科学与工程学院,天津300072

出　　处：《传感技术学报》2007年第2期258-261,共4页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助(20576092)

摘　　要：在制备由聚乙烯醇和二氧化硅溶胶凝胶掺杂而成的有机-无机杂化材料的基础上,将葡萄糖氧化酶用此杂化材料包埋固定在铂盘电极上,利用修饰电极对过氧化氢的电催化还原特性,制得了高性能的安培型葡萄糖氧化酶电极.此酶电极可以在0.05V(vsSCE)下对葡萄糖进行测定,有效地消除了抗坏血酸和尿素的干扰.最适pH值为7.0,载酶量为6%.在选定的工作条件下,此酶电极的灵敏度和线性范围分别为211nA/mmol.L-1和0.1～4.0mmol.L-1,平均响应时间为2.8s左右.An organic-inorganic hybrid material based on sol-gel derived SiO2 and PVA has been obtained. Glucose oxidase is immobilized by this hybrid material on platinum electrode surface which can catalyze the reduction of hydrogen peroxide and an amperometric glucose biosensor of high performance has been fabricated. The developed sensor is applied to detect glucose with operating potential of 0.05 V（vs SCE）, which eliminates the interferences of ascorbic acid and uric acid effectively. Optimum pH is 7.0 and enzymes loading is 6%. Under selective conditions the biosensor shows excellent sensitivity of 211 nA/mmol/L and a linear range of 0.1-4.0 mmol·L^-1. The average response time is 2. 8 s.

关 键 词：葡萄糖传感器 修饰电极 溶胶-凝胶 聚乙烯醇 杂化材料 铂盘电极 

分 类 号：O657.1[理学—分析化学] TP212.2[理学—化学]