纳米TiO_2和纳米Al_2O_3对小鼠血清乳酸脱氢酶和α-羟丁酸脱氢酶活力的影响  

EFFECT OF EXCESS NANO-PARTICLE ALUMINA OXIDE AND TITANIUM DIOXIDE ON SERUM LDH AND α-HBDH ACTIVITY IN MICE

作　　者：王天成[1] 贾光[2] 王翔[2] 陈春英[3] 孙红芳[4] 沈慧麒[2] 赵宇亮[3] 

机构地区：[1]北京大学第三医院检验科,北京100083 [2]中国科学院高能物理研究所 [3]北京大学医学部公共卫生学院劳动卫生与环境卫生学系 [4]北京大学化学与分子工程学院化学生物学系

出　　处：《现代预防医学》2007年第3期405-406,共2页Modern Preventive Medicine

基　　金：国家十五重大项目(10490180)

摘　　要：[目的]探讨大剂量经口纳米粒径二氧化钛(nano-TiO2)和三氧化二铝(nano-Al2O3)粉染毒对小鼠血清乳酸脱氢酶(LDH)和α-羟丁酸脱氢酶(α-HBDH)活力的影响。[方法]分别以5g/Kg体重剂量的微米(micro-TiO2)和nano-TiO2或-Al2O3粉给小鼠一次经口灌胃,14d后处死取血分离血清,分析LDH和α-HBDH活性变化。[结果]nano-Al2O3染毒小鼠血清LDH和α-HBDH活力比对照组明显降低(P﹤0.05),但与micro-Al2O3染毒组相比差异无统计学意义(P﹥0.05);nano-TiO2染毒小鼠血清LDH活力比对照组有明显升高(P﹤0.05),micro-TiO2染毒组小鼠血清LDH活力明显低于对照组和nano-TiO2染毒组(P﹤0.05);nano-TiO2染毒小鼠血清α-HBDH活力与micro-TiO2染毒组相比差异无统计学意义(P﹥0.05)。[结论]nano-TiO2和nano-Al2O3粉对小鼠血清LDH和α-HBDH活性的影响明显不同,nano-TiO2和nano-Al2O3粉对小鼠血清LDH和α-HBDH活性的影响与相应的micro-TiO2和micro-Al2O3有明显差别,血清LDH和α-HBDH可能不一定适合于纳米金属材料的生物毒性评估。[Objective] To explore the effect of excess nano-particle alumina oxide and titanium dioxide on serum Lactate dehydrogenase （LDH） and α-Hydroxybutyrate Dehydrogenase （α-HBDH） activity in mice. [Methods] The Mice were treated with micro-or nano-particle alumina oxide or titanium dioxide powder at a single oral dose of 5 g/Kg body weight. 14 days later, the mice were sacrificed and the serum was collected for LDH and α-HBDH analysis. [ Results] The serum LDH and HBDH activity in Nano-Al2O3 group mice were lower significantly than that in control group but without obvious difference with that in micro-Al2O3 group mice. The serum LDH activity of nano-TiO2 group mice was significandy higher than that of control group mice, but there was no any difference in serum HBDH activity between micro-and nano-TiO2 group mice. The serum LDH activity in micro-TiO2 group mice decreased significandy than control group and nano-TiO2 group; there was no any difference in serum HBDH activity among the control, nano- and micro-TiO2, group mice. [Conclusions] There are significant different effects on serum LDH and α-HBDH between excess Nano-Al2O3 and Nano-TiO2 treated mice, excess nano-Al2O3 or nano-TiO2 has obvious different effect on serum LDH and α-HBDH than micro-Al2O3 or micro-TiO2, serum LDH and HBDH activity are probably not suitable for the evaluation on the biological injury and toxic of nano metal materials.

关 键 词：纳米 钛 铝 血清 乳酸脱氢酶 Α-羟丁酸脱氢酶 小鼠 

分 类 号：TB383[一般工业技术—材料科学与工程] R114[医药卫生—卫生毒理学]