全反式维甲酸对甲状腺鳞癌细胞株SW579凋亡的影响  

Influences of All-trans-retinoic Acid on Apoptosis of Thyroid Squamous Cell Carcinoma Cell Line SW579

作　　者：张秀梅[1] 肖建英[1] 孙靖靖[1] 岳丹[1] 

机构地区：[1]锦州医学院生物化学教研室,辽宁锦州121000

出　　处：《锦州医学院学报》2006年第6期5-7,共3页Journal of Jinzhou Medical College

基　　金：辽宁省自然科学基金项目(编号20062001)

摘　　要：目的观察全反式维甲酸(all-trans-retinoic acid,ATRA)体外诱导甲状腺鳞癌细胞株SW579形态学变化和细胞凋亡。方法分别以终浓度为10-7、10-6、10-5、2×10-5、4×10-5、6×10-5、8×10-5、10-4mol/L的维甲酸作用SW 579细胞株,在倒置显微镜下观察细胞生长情况及形态变化;利用吖啶橙染色,荧光显微镜下观察细胞凋亡;流式细胞仪检测细胞凋亡率。结果ATRA作用后,可见细胞形态改变;经荧光染色后可见凋亡的细胞,染色质浓集,呈致密的斑块状或新月状,并可见凋亡小体及核碎片;流式细胞仪分析,凋亡率随ATRA浓度的升高而升高(P<0.01)。结论不同浓度的ATRA可诱导SW 579细胞形态学变化及细胞凋亡。Objective To observe the impacts of all - trans - retinoic acid on cell morphologic and apoptosis of thyroid cancer cell line （SW579） in vitro. Methods Treated with different dose of ATRA （final concentration of 10^-7、10^-6、10^-5、2×10^-5、4×10^-5、6×10^-5、8×10^-5、10^-4moL/L） , the cells were observed with a convert microscope in shape. The apoptosis was evaluated by AQ -stained fluorescin, and apoptosis index was measured by flow cytometry. Results SW579 ceils treated with ATRA at different concentration underwent apoptosis and presented apoptotic feature： Chromatin condensation, nuclei fragment and apoptotic body formation, flow cytometry analysis showed ATRA increased apoptosis index with the concentration from 10^-7 to 8 × 10^-5. Conclusions ATRA can induce morphological changes and apoptosis in the thyroid cancer cell line.

关 键 词：全反式维甲酸 甲状腺鳞癌 细胞凋亡 

分 类 号：R979.1[医药卫生—药品]