皮质醇对TSH调节猪甲状腺细胞钠/碘转运体的影响作用  

Effect of Cortisol on the Ability of Thyrotropin to Regulate the Sodium/iodide Symporter of Porcine Thyroid Cells

作　　者：秦昕煜[1] 曲莎娜[1] 刘用璋[1] 孙浩[2] 周明会[2] 

机构地区：[1]锦州医学院生物化学教研室 [2]解放军第205医院核医学科,辽宁锦州121001

出　　处：《锦州医学院学报》2006年第6期11-13,共3页Journal of Jinzhou Medical College

摘　　要：目的研究皮质醇在促甲状腺激素（TSH）调节甲状腺细胞功能中的作用。方法采用细胞原代培养技术，检测不同浓度皮质醇（HC）对TSH刺激的甲状腺细胞摄碘能力和Na^＋-K^＋-ATP酶活力的影响。结果皮质醇在10—500nmo]／L浓度时可促进1U／L的TSH刺激的甲状腺细胞对碘的摄取，增强Na^＋-K^＋-ATP酶的活力，且成剂量依赖性；但当皮质醇的浓度达到一定范围（1000nmo]／L），其对TSH效应的促进作用不再随着浓度的升高而增强。结论皮质醇与TSH对甲状腺细胞钠／碘转运体（NIS）功能具有协同促进作用。Objective To investigate the effect of cortisol on the ability of thyrotropin to regulate the function of porcine thyroid cells. Methods Thyroid cells obtained from porcine were cultured in a monolayer system. The uptake of iodide assay was used to detect the function of NIS in normal thyroid cells. Changes of the Na^＋-K^＋-ATPase were observed, which related to NIS. Results Cortisol in 10 500nM can encourage TSH - increased the iodide uptake and Na^＋-K^＋-- ATPase vigor of thyroid cell in dose -dependent manner, but when the dosage reached certain density （1000nM） , this promotion no longer strengthened along with the increase of density. Conclusions Cortisol and TSH have synergistic effect with thyrotropin.

关 键 词：皮质醇 甲状腺 细胞培养 钠/碘转运体 

分 类 号：R977[医药卫生—药品]