汞暴露对草鱼器官组织中碱性磷酸酶活性的影响  

Effects of Hg^(2+) on alkaline phosphatase activity in different organs and tissues of grass carp(Ctenopharyngodon idella)

作　　者：孔祥会[1] 刘占才[1] 郭彦玲[1] 郭春丽[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《中国水产科学》2007年第2期270-274,共5页Journal of Fishery Sciences of China

基　　金：河南省科技攻关项目(0624030022);河南省动物学重点学科资助项目

摘　　要：测定暴露于不同汞离子质量浓度(0 mg/L、0.05 mg/L、0.10 mg/L、0.15 mg/L、0.20 mg/L、0.25 mg/L)下草鱼(Ctenopharyngodon idella)血清、鳃、肝胰脏、脾、肾脏和肌肉等组织器官中碱性磷酸酶(alkaline phosphatase,AKP)活性。研究目的在于评价汞离子对草鱼生理代谢的影响。暴露周期为21 d。结果显示,与对照组相比,血清AKP活性在0.05mg/L、0.10 mg/L和0.15 mg/L汞暴露组无显著性变化(P>0.05),而在0.20 mg/L、0.25 mg/L组显著下降(P<0.01);鳃AKP活性在0.10 mg/L和0.15 mg/L汞暴露组显著上升(P<0.05或P<0.01),而0.05 mg/L、0.20 mg/L和0.25mg/L组无显著变化(P>0.05);肝胰脏和脾脏AKP活性在所有实验组均显著下降(P<0.01);肾脏AKP活性在0.05mg/L组无显著变化,其他实验组均显著升高(P<0.01);肌肉AKP活性在所有实验组均无显著性变化(P>0.05)。本研究说明草鱼暴露于不同汞离子浓度下。Grass carp Ctenopharyngodon idella is sensitive to environmental changes in water. As environmental factors fluctuate, physiological metabolism will change correspondingly. Alkaline phosphatase is a kind of important regulating enzyme for metablism. To study the change of alkaline phosphatase（AKP） activity can help understand the effects of environmental factors on physiological metabolism of fish. In this study,grass carp were randomly devided into 6 groups（5 experimental groups and one control group, each group with 10 fish）. Experimental groups were exposed to different Hg^2＋ concentrations（0.05 mg/L, 0.10 mg/L,0.15 mg/L,0.20 mg/L, 0.25 mg/L）. On day 21, serum, gill, liver, spleen, kidney and muscle were sampled to analyse. Compared with the control, AKP activity in serum showed no significant change at Hg^2 ＋ concentrations of 0.05 mg/L, 0.10 mg/L and 0.15 mg/L（P 〉0.05 ）, while it decreased significantly at 0.20 mg/L and 0.25 mg/L（P〈0.01）. For gill,AKP activity increased significantly only at 0.10 mg/L （P〈0.05） and 0.15 mg/L（P〈0.01） ,and did not change obviously in the other groups（P 〉0.05）. AKP activities in liver and spleen gradually decreased with the increment of Hg^2＋ concentration, and were significantly lower than that in control（P 〈 0.01 ）. The activity of AKP in kidney showed no obvious change in 0.05 mg/L group（ P 〉0.05 ）, while enzyme activities in the other experimental groups were significantly higher than that in control（P〈0.01 ）. For muscle, AKP activity showed no significant changes at various experimental concentration（P 〉 0.05）. In summary, when grass carp was exposed to various Hg^2 ＋ concentrations,AKP activities in different organs and tissues, which perform different physiological functions, appear to be different. AKP activity in liver and spleen can be used as potential indicators to evaluate Hg^2＋ exposure effects.

关 键 词：汞离子 碱性磷酸酶 草鱼 

分 类 号：Q55[生物学—生物化学]