反相高效液相色谱法分离中华绒螯蟹性腺抑制激素的初步研究  

Primary study of GIH isolation in Eriocheir sinensis by RP-HPLP

作　　者：马奎[1] 康现江[1] 穆淑梅[1] 张亮[1] 温秀荣[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《中国水产科学》2007年第2期331-335,共5页Journal of Fishery Sciences of China

基　　金：河北省自然科学基金资助项目(303118);河北大学青年科学基金资助项目(2005Q13)

摘　　要：采用反相高效液相色谱层析法(RP-HPLC)分离中华绒螯蟹(Eriocheir sinensis)眼柄视上神经节中的性腺抑制激素,收集在高血糖激素家族区域内出峰的洗脱液组分,得到7组单一的样品组分。通过活体注射的方法,观察和统计每一组分对去眼柄中华绒螯蟹卵母细胞直径大小的影响,以检验每个分离组分的性腺抑制活性。结果发现,其中的第7组分对中华绒螯蟹的卵巢发育具有最强的抑制作用,推测这一组分为中华绒螯蟹的性腺抑制激素。The gonad inhibiting hormone （GIH） is a member of Crustacean hyperglycemic hormone （CHH） peptide family. It plays a significant role in inhibiting development of crab＇s gonad. In this research, the crude hormone extract of crab＇s optic ganglia was further isolated by reverse-phase high-performance liquid chromatography （RP-HPLC）. Seven single fractions were obtained. The biological activity of each fraction was assayed through injecting in vivo to the crabs whose optic ganglia were removed. After 6 days of injection the diameter of crab＇ s oocytes was recorded and statistically analyzed. The results showed that among the 7 isolated fractions, fraction 7 had the greatest biological activity to suppress the development of crab oocytes,which indicated that this fraction was the GIH of Eriooheir sinensis.

关 键 词：中华绒螯蟹 性腺抑制激素 反相高效液相色谱 卵母细胞 

分 类 号：Q959.223[生物学—动物学]