五色灵芝对人鼻咽癌细胞株在裸鼠成瘤的抑制作用  

Effect of Wild LINGZHI Oral Extract on Suppression of Growth of Human NasopharyngealCarcinoma Cells

作　　者：陈小君[1] 陈剑经[1] 李亮平[1] 孙艳 

机构地区：[1]中山医科大学肿瘤研究所

出　　处：《实验动物科学与管理》1996年第3期27-29,共3页Laboratory Animal Science & Administration

摘　　要：本项研究用五种野生灵芝提取物混合液（口服液），用９ｇ／ｋｇ／ｄ的剂量，给接种人鼻咽低分化鳞状上皮癌细胞株（每只棵鼠接种２×１０￣６细胞数）３天后的裸鼠灌胃给药，对照组用生理盐水２０ｍｌ／ｋｇ／ｄ，两组均按上述剂量分别灌胃２０天，裸鼠饲养于温度２５℃。相对温度为５０％～７０％的无菌室内。定期观察各组裸鼠的生长及成瘤情况，实验结果时将动物处死，称瘤重、鼠重；并计算其抑瘤率，取瘤组织肺、肝做组织切片。结果表明五色灵芝口服液对人鼻咽癌细胞株在裸鼠成瘤有明显的抑制作用，经三次试验证实肿瘤的抑制率在３８．５～４４．２％之间，均Ｐ＜０．００１，各组裸鼠用药后体重均有较明显增加，其增长幅度与对照组相比基本无差异，各组小鼠在实验中一般状态良好，在本实验的条件下未见本药有毒副作用。We have investigated the growth of human nasopharyngeal carcinoma(NPC)cells in nude mice three days af-ter the intragastric infusion of the mixed extract of 5 types of wild LINGZHI.The maimals had been inoculated with cellstaken from human poorly diffcrentiated nasopharyngcal squamous cpitheliocarcinoma ccll line. The number of cells used in in-orulation was 2×106 for cach animal.The dosc of LINGZHl was 9g/kg/d as comparcd to that of normal salinc,20ml/kg/d,in the control group intragastric infusion were given consccutively for 20days in both groups. The room,in which nude micewere grown,was sterilized with a temperature of 25℃ and a relative humidity of 50～ 70%。 After regular observations,theanimals were killed and weighed.The weights of he tumors were also noted down,the tumor inhibitory rate calculatcd andsecretions of the lung and liver examined histologically. The results revealed that wild LINGZHI oral extract may have amarked effect on the tumor formation in human NPC cell Iine. As seen in the results of the three series of our tests,the tumorsuppression rate of LINGZHI was 38.5～44.2% with P<0.001. Body weights of nude mice in all experimental groups in-creased significantly to an extent approximating that of the control groups. The general state of mice in various groups ap-peared normal throghout the study with no toxic side reaction under our experimental condition.

关 键 词：中药 灵芝 五色灵芝 鼻咽肿瘤 癌 裸鼠 成瘤 

分 类 号：R282.710.7[医药卫生—中药学] R273.963[医药卫生—中医学]