维生素C保护糖尿病大鼠主动脉的作用机制  

Protective Mechanism of Vitamin C on Aorta in Diabetic Rats

作　　者：高华[1] 胡建国[1] 张卓[2] 李剑欣[2] 盛杰[3] 刘锋[3] 李强翔[2] 

机构地区：[1]中南大学湘雅二医院心胸外科,长沙410011 [2]湖南省娄底市中心医院 [3]中南大学湘雅医院内分泌科

出　　处：《天津医药》2007年第2期121-123,共3页Tianjin Medical Journal

基　　金：湖南省卫生厅资助项目(项目编号:B2006215)

摘　　要：目的:观察维生素C对糖尿病大鼠血脂和主动脉纤维连接蛋白(FN)表达的影响。方法:利用链脲佐菌素腹腔注射法诱导建立1型糖尿病大鼠模型,将实验用SD大鼠随机分正常对照组、造模组(包括糖尿病组和维生素C治疗组)。治疗16周,观察治疗期间及治疗后大鼠的一般状况、血糖(BG)、三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)、糖化血红蛋白(HbA1c)和糖化低密度脂蛋白(G-LDL)。大鼠麻醉处死,分离主动脉,HE染色及免疫组织化学检测主动脉内膜FN表达。结果:造模组大鼠均出现血脂异常及主动脉病理形态改变。维生素C对血糖无影响,但能降低糖尿病大鼠的三酰甘油、胆固醇、LDL、HbA1c、G-LDL,升高HDL,且能降低主动脉纤维连接蛋白表达。结论:维生素C无降糖作用,但可通过调节血脂,抑制主动脉纤维连接蛋白表达对糖尿病大鼠主动脉起到保护作用。Objective： To observe the effect of Vitamin C （Vit C） on blood lipid and expression of aorta fibronectin in diabetic rats. Methods： Diabetic model was induced by intraperitoneal injection of streptozotocin （STZ） in rats. Rats were divided into normal control group, model group and Vit C group. During and after 16 weeks of Vit C treatment, the levels blood glucose （BG）, triglyceride （TC）, cholesterol, low density lipoprotein （LDL）, high density lipoprotein （HDL）, glycosylated hemoglobin （HbA1c） and glycosylated low density lipoprotein （G-LDL） were measured. Rats were killed at the end of experiments and specimens from aorta were taken and observed with HE stain. The expression of fibronectin was determined by immunohistochemistry. Results： Abnormal blood lipids and aorta lesion were found in two model groups. Vit C had no effect on the blood glucose levels. Vit C decreased TC, serum cholesterol, LDL-C, HbA1c and G-LDL and increase HDL-C, while the expression of aorta fibronectin was significantly inhibited at the same time. Conclusion： Vit C has no effect on decreasing the blood glucose level but has certain protective effect on aorta by inhibiting aorta expression of fibronectin and adjusting blood lipid.

关 键 词：糖尿病 1型 抗坏血酸 疾病模型 动物 脂类 主动脉 纤维蛋白 

分 类 号：R33[医药卫生—人体生理学]