DC2.4表面分子与RelB基因表达的关系  

Relationship between surface molecules and RelB gene expression in DC2.4 cell line

作　　者：包杰[1] 郑磊[1] 曾方银[1] 杨红玲[1] 裘宇容[1] 李娟[2] 王前[1] 

机构地区：[1]南方医科大学南方医院检验医学中心,广东广州510515 [2]南方医科大学南方医院风湿科,广东广州510515

出　　处：《南方医科大学学报》2007年第1期56-58,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(04020404)~~

摘　　要：目的探讨DC2.4表面分子表达水平与RelB基因表达间的关系。方法用RPMI1640完全培养液培养DC2.4细胞系,光学显微镜观察培养细胞的形态特征;透射电镜观察DC2.4的细胞结构;流式细胞术分析DC2.4的表面标记(MHC-II、CD86和CD40);RT-PCR检测DC2.4的RelB表达水平。结果光镜观察DC2.4细胞表面有明显的树突状突起,形态极不规则;透射电镜显示DC2.4细胞内含许多质地均匀的脂滴,可见吞噬小泡样的结构。流式细胞术显示MHC-II类分子和CD40呈低水平表达,而CD86分子却呈高水平表达;RT-PCR结果显示RelB呈低水平表达,与骨髓源性DC中的未成熟状态DC的RelB表达水平接近。结论DC2.4具有强吞噬功能,其表面CD40分子和细胞内RelB基因均呈低水平表达,提示DC2.4是一种未成熟状态的细胞系。Objective To explore the relationship between the expression of the nuclear factor-kappaB transcription factor RelB gene and the surface molecules in DC2.4 cell line. Methods DC2.4 cell line was cultured in complete RPMI 1640 medium, whose morphology was observed with optical microscope and the intracellular structures with transmission electron microscope. Flow cytometry was performed to analyze the surface markers of the cells, including MHC-Ⅱ, CD86 and CD40, and RelB mRNA expression was detected by RT-PCR. Results Under optical microscope, the cells appeared irregular in shape with obvious dendritic cell processes, and electron microscopy revealed homogenous fat drops and phagocytic vesicles in the cytoplasm. Flow cytometry demonstrated low expression levels of MHC-Ⅱ and CD40, but high level of CD86 molecules. Low-level expression of RelB mRNA was detected by RT-PCR, resembling its expression level in bone marrow-derived DC with immature phenotype. Conclusion DC2.4 is a mouse bone marrow dendritic cell line with strong phagocytic capacity, and the low expression of both RelB gene and surface CD40 molecules suggests an immature dendritic cell line.

关 键 词：DC2.4 RELB 基因表达 

分 类 号：R392.12[医药卫生—免疫学]