GC盒不是人胰岛素基因启动子中关键顺式作用元件  

GC-box is not a key cis-acting element in human insulin gene promoter

作　　者：牛新捷[1,2] 王作仁[1] Jochen Seufert 

机构地区：[1]西安交通大学第一医院肝胆外科 [2]德国弗莱堡大学医学院内分泌和糖尿病科,弗莱堡79095

出　　处：《南方医科大学学报》2007年第1期78-80,共3页Journal of Southern Medical University

基　　金：德国教育与科研部基金(FKZ01GN0115)~~

摘　　要：目的鉴定人胰岛素基因启动子中的GC盒(-348￣-338)是否是启动子中的关键顺式作用元件。方法用PCR克隆人胰岛素基因启动子,构建由人胰岛素基因启动子驱动的分泌型碱性磷酸酶(SEAP)报告质粒,并对报告质粒中人胰岛素基因启动子中的GC盒进行删除和突变。将报告质粒转染到!细胞系"TC3中,测定细胞培养液上清中SEAP的活性强度,进而判定GC盒与人胰岛素基因启动子转录活性的关系。结果删除和突变人胰岛素启动子中的GC盒不能显著改变胰岛素基因启动子在#细胞中的转录活性。结论人胰岛素基因启动子中GC盒不是一个关键顺式作用元件。Objective To identify whether GC-box （-348 to -338） in human insulin gene promoter is a key cis-acting element. Methods Human insulin gene promoter was sub-cloned into secreted alkaline phosphatase （SEAP） reporter plasmid. The deletion and mutation of GC-box in insulin gene promoter was performed. The activity of human insulin gene promoter was determined by evaluating the activity of SEAP in the supematant of cell culture after the reporter plasmids were transfected in beta cell line βTC3. Result Deletion and mutation of GC box in human insulin gene promoter did not result in significant changes of the activity of the promoter in βTC3. Cohclusion The GC-box is not a key cis-acting element in human insulin gene promoter.

关 键 词：胰岛素基因 GC盒 启动子 分泌型碱性磷酸酶 顺式作用元件 

分 类 号：R346[医药卫生—基础医学]