低温对转抗冻蛋白基因罗非鱼影响的初步研究  

The Influence of Low Temperature on Tilapia of Transferred Antifreeze Protein Gene

作　　者：李晶[1] 李荣萍[1] 张淑梅[1] 赵晓祥[1] 张云湖[1] 杨志兴[1] 

机构地区：[1]黑龙江省科学院应用微生物所

出　　处：《生物技术》1996年第5期9-10,共2页Biotechnology

基　　金：黑龙江省"八五"攻关项目

摘　　要：本文阐述了逐渐降低水温对转抗冻蛋白基因罗非鱼生存影响的初步观察结果。用３０尾转抗冻蛋白基因罗非鱼，从２２℃开始，以２℃／ｈ下降至７℃，发现在９℃有６尾死亡，７℃又有１０尾死亡，有１４尾存活，但有不同表现，从存活１４尾鱼和死亡的６尾鱼中提取总ＤＮＡ，作原位杂交，有１０尾为阳性，再从这１０尾呈阳性鱼肝中分离总ＲＮＡ，作ＲＮＡ—ＤＮＡ杂交并未发现阳性结果，表明有外源ＤＮＡ的整合，但未发生转录或弱的转录，不过有部分转基因鱼表现出具有耐低温能力。We reported the result that affected the survival of Tilapia of transferred antifreeze protein gene by reducing the water temperature gradually.For 30'transgenic Tilapia,we reduced the water temperature from 22℃ to 7℃ by 2℃ an hour.At 9℃,6 fish were found dead and 10 fish died at 7C,14 fish were alive and they had different character.DNA was extracted from 14 living fish and 6 dead fish,and situ hybridization showed 10 fish were positive.We also extractedliver RNA of 10 positive fish and Northern blotting was negative.The result indicated that foreign DNA was intergrated into the genome of Tilapia and no transmission or weak transmission was found.But some of transgenic fish were resistant to low temperature.

关 键 词：罗非鱼 转基因鱼 低温 转抗冻蛋白基因 

分 类 号：Q959.483[生物学—动物学]